Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7bkb
Q-score: 0.348
Watanabe T, Pfeil-Gardiner O, Kahnt J, Koch J, Shima S, Murphy BJ
Science (2021) 373 pp. 1151-1156 [ Pubmed: 34516836 DOI: doi:10.1126/science.abg5550 ]
- Formylmethanofuran dehydrogenase (28 kDa, Protein from Methanospirillum hungatei JF-1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cob--com heterodisulfide reductase iron-sulfur subunit a (72 kDa, Protein from Methanospirillum hungatei JF-1)
- Non-cubane [4fe-4s]-cluster (351 Da, Ligand)
- Formate dehydrogenase, beta subunit (f420) (45 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit a (63 kDa, Protein from Methanospirillum hungatei JF-1)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Formate dehydrogenase (75 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit b (32 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit g (16 kDa, Protein from Methanospirillum hungatei JF-1)
- 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide (740 Da, Ligand)
- Molybdenum atom (95 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dimeric formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex (948 kDa, Complex from Methanospirillum hungatei JF-1)
- F420-non-reducing hydrogenase subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit c (21 kDa, Protein from Methanospirillum hungatei JF-1)
- Iron/sulfur cluster (351 Da, Ligand)
- Formylmethanofuran dehydrogenase, subunit f (42 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit b (49 kDa, Protein from Methanospirillum hungatei JF-1)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7bkc
Q-score: 0.569
Watanabe T, Pfeil-Gardiner O, Kahnt J, Koch J, Shima S, Murphy BJ
Science (2021) 373 pp. 1151-1156 [ Pubmed: 34516836 DOI: doi:10.1126/science.abg5550 ]
- Formylmethanofuran dehydrogenase (28 kDa, Protein from Methanospirillum hungatei JF-1)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cob--com heterodisulfide reductase iron-sulfur subunit a (72 kDa, Protein from Methanospirillum hungatei JF-1)
- Non-cubane [4fe-4s]-cluster (351 Da, Ligand)
- Formate dehydrogenase, beta subunit (f420) (45 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit a (63 kDa, Protein from Methanospirillum hungatei JF-1)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Formate dehydrogenase (75 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit b (32 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit g (16 kDa, Protein from Methanospirillum hungatei JF-1)
- 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide (740 Da, Ligand)
- Molybdenum atom (95 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dimeric formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex (948 kDa, Complex from Methanospirillum hungatei JF-1)
- F420-non-reducing hydrogenase subunit d (15 kDa, Protein from Methanospirillum hungatei JF-1)
- Cob--com heterodisulfide reductase subunit c (21 kDa, Protein from Methanospirillum hungatei JF-1)
- Iron/sulfur cluster (351 Da, Ligand)
- Formylmethanofuran dehydrogenase, subunit f (42 kDa, Protein from Methanospirillum hungatei JF-1)
- Formylmethanofuran dehydrogenase, subunit b (49 kDa, Protein from Methanospirillum hungatei JF-1)
Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - Fo-subcomplex
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6ynx
Q-score: 0.617
Flygaard RK, Muhleip A, Tobiasson V, Amunts A
Nat Commun (2020) 11 pp. 5342-5342 [ Pubmed: 33093501 DOI: doi:10.1038/s41467-020-18993-6 ]
- Atptt11 (17 kDa, Protein from Tetrahymena thermophila)
- Atptt7 (26 kDa, Protein from Tetrahymena thermophila)
- Subunit i/j (24 kDa, Protein from Tetrahymena thermophila)
- Subunit d (26 kDa, Protein from Tetrahymena thermophila)
- Atptt10 (17 kDa, Protein from Tetrahymena thermophila)
- Inhibitor of f1 (if1) (12 kDa, Protein from Tetrahymena thermophila)
- Atptt2 (54 kDa, Protein from Tetrahymena thermophila)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (749 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Atptt4 (32 kDa, Protein from Tetrahymena thermophila)
- Atptt6 (29 kDa, Protein from Tetrahymena thermophila)
- Mitochondrial atp synthase, fo-subcomplex (Complex from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atptt1 (54 kDa, Protein from Tetrahymena thermophila)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Atptt8 (21 kDa, Protein from Tetrahymena thermophila)
- Atptt9 (18 kDa, Protein from Tetrahymena thermophila)
- Cardiolipin (1 kDa, Ligand)
- Atptt3 (34 kDa, Protein from Tetrahymena thermophila)
- Subunit k (20 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Subunit b (43 kDa, Protein from Tetrahymena thermophila)
- Subunit a (52 kDa, Protein from Tetrahymena thermophila)
- Ubiquinone-8 (727 Da, Ligand)
- Atptt13 (16 kDa, Protein from Tetrahymena thermophila)
- Subunit f (24 kDa, Protein from Tetrahymena thermophila)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Atptt5 (31 kDa, Protein from Tetrahymena thermophila)
- Subunit 8 (12 kDa, Protein from Tetrahymena thermophila)
- Atptt12 (17 kDa, Protein from Tetrahymena thermophila)
Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1Fo dimer
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6yny
Q-score: 0.403
Flygaard RK, Muhleip A, Tobiasson V, Amunts A
Nat Commun (2020) 11 pp. 5342-5342 [ Pubmed: 33093501 DOI: doi:10.1038/s41467-020-18993-6 ]
- Atptt11 (17 kDa, Protein from Tetrahymena thermophila)
- Atptt7 (26 kDa, Protein from Tetrahymena thermophila)
- Subunit i/j (24 kDa, Protein from Tetrahymena thermophila)
- Subunit d (26 kDa, Protein from Tetrahymena thermophila)
- Atptt10 (17 kDa, Protein from Tetrahymena thermophila)
- Inhibitor of f1 (if1) (12 kDa, Protein from Tetrahymena thermophila)
- Subunit alpha (59 kDa, Protein from Tetrahymena thermophila)
- Subunit delta (17 kDa, Protein from Tetrahymena thermophila)
- Atptt2 (54 kDa, Protein from Tetrahymena thermophila)
- Oligomycin sensitivity-conferring protein (oscp) (24 kDa, Protein from Tetrahymena thermophila)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (749 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atptt4 (32 kDa, Protein from Tetrahymena thermophila)
- Atptt6 (29 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atptt1 (54 kDa, Protein from Tetrahymena thermophila)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Atptt8 (21 kDa, Protein from Tetrahymena thermophila)
- Atptt9 (18 kDa, Protein from Tetrahymena thermophila)
- Cardiolipin (1 kDa, Ligand)
- Subunit gamma (32 kDa, Protein from Tetrahymena thermophila)
- Atptt3 (34 kDa, Protein from Tetrahymena thermophila)
- Subunit k (20 kDa, Protein from Tetrahymena thermophila)
- Mitochondrial atp synthase, f1fo dimer (Complex from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Subunit b (43 kDa, Protein from Tetrahymena thermophila)
- Subunit c (8 kDa, Protein from Tetrahymena thermophila)
- Subunit a (52 kDa, Protein from Tetrahymena thermophila)
- Subunit beta (53 kDa, Protein from Tetrahymena thermophila)
- Ubiquinone-8 (727 Da, Ligand)
- Atptt13 (16 kDa, Protein from Tetrahymena thermophila)
- Subunit f (24 kDa, Protein from Tetrahymena thermophila)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Atptt5 (31 kDa, Protein from Tetrahymena thermophila)
- Subunit 8 (12 kDa, Protein from Tetrahymena thermophila)
- Subunit epsilon (7 kDa, Protein from Tetrahymena thermophila)
- Atptt12 (17 kDa, Protein from Tetrahymena thermophila)
Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1Fo tetramer
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6ynz
Q-score: 0.317
Flygaard RK, Muhleip A, Tobiasson V, Amunts A
Nat Commun (2020) 11 pp. 5342-5342 [ Pubmed: 33093501 DOI: doi:10.1038/s41467-020-18993-6 ]
- Atptt11 (17 kDa, Protein from Tetrahymena thermophila)
- Atptt7 (26 kDa, Protein from Tetrahymena thermophila)
- Subunit i/j (24 kDa, Protein from Tetrahymena thermophila)
- Atp synthase subunit beta (53 kDa, Protein from Tetrahymena thermophila)
- Subunit d (26 kDa, Protein from Tetrahymena thermophila)
- Atptt10 (17 kDa, Protein from Tetrahymena thermophila)
- Inhibitor of f1 (if1) (12 kDa, Protein from Tetrahymena thermophila)
- Subunit delta (17 kDa, Protein from Tetrahymena thermophila)
- Atptt2 (54 kDa, Protein from Tetrahymena thermophila)
- Oligomycin sensitivity-conferring protein (oscp) (24 kDa, Protein from Tetrahymena thermophila)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (749 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atp synthase f0 subunit 9 (8 kDa, Protein from Tetrahymena thermophila)
- Atptt4 (32 kDa, Protein from Tetrahymena thermophila)
- Atptt6 (29 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atptt1 (54 kDa, Protein from Tetrahymena thermophila)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Atptt8 (21 kDa, Protein from Tetrahymena thermophila)
- Ymf66 (52 kDa, Protein from Tetrahymena thermophila)
- Atptt9 (18 kDa, Protein from Tetrahymena thermophila)
- Cardiolipin (1 kDa, Ligand)
- Atptt3 (34 kDa, Protein from Tetrahymena thermophila)
- Subunit k (20 kDa, Protein from Tetrahymena thermophila)
- Atp synthase subunit gamma (32 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Subunit b (43 kDa, Protein from Tetrahymena thermophila)
- Mitochondrial atp synthase, f1fo tetramer (Complex from Tetrahymena thermophila)
- Atp synthase subunit alpha (59 kDa, Protein from Tetrahymena thermophila)
- Ubiquinone-8 (727 Da, Ligand)
- Atptt13 (16 kDa, Protein from Tetrahymena thermophila)
- Subunit f (24 kDa, Protein from Tetrahymena thermophila)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Atptt5 (31 kDa, Protein from Tetrahymena thermophila)
- Subunit epsilon (7 kDa, Protein from Tetrahymena thermophila)
- Ymf56 (12 kDa, Protein from Tetrahymena thermophila)
- Atptt12 (17 kDa, Protein from Tetrahymena thermophila)
Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1/peripheral stalk
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6yo0
Q-score: 0.512
Flygaard RK, Muhleip A, Tobiasson V, Amunts A
Nat Commun (2020) 11 pp. 5342-5342 [ Pubmed: 33093501 DOI: doi:10.1038/s41467-020-18993-6 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Atp synthase subunit alpha (59 kDa, Protein from Tetrahymena thermophila)
- Atptt13 (16 kDa, Protein from Tetrahymena thermophila)
- Atp synthase subunit beta (53 kDa, Protein from Tetrahymena thermophila)
- Subunit d (26 kDa, Protein from Tetrahymena thermophila)
- Atp synthase subunit gamma (32 kDa, Protein from Tetrahymena thermophila)
- Oligomycin sensitivity-conferring protein (oscp) (24 kDa, Protein from Tetrahymena thermophila)
- Inhibitor of f1 (if1) (12 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Subunit b (43 kDa, Protein from Tetrahymena thermophila)
- Mitochondrial atp synthase, f1/peripheral stalk (Complex from Tetrahymena thermophila)
Resolution: 3.61 Å
EM Method: Single-particle
Fitted PDBs: 6zbb
Q-score: 0.45
Spikes TE, Montgomery MG, Walker JE
PNAS (2020) 117 pp. 23519-23526 [ Pubmed: 32900941 DOI: doi:10.1073/pnas.2013998117 ]
- Atp synthase subunit g, mitochondrial (11 kDa, Protein from cattle)
- Atp synthase f(0) complex subunit b1, mitochondrial (24 kDa, Protein from cattle)
- Atp synthase f(0) complex subunit c1, mitochondrial (7 kDa, Protein from cattle)
- Atp synthase subunit a (24 kDa, Protein from cattle)
- Atp synthase protein 8 (7 kDa, Protein from cattle)
- Atp synthase subunit e, mitochondrial (8 kDa, Protein from cattle)
- Atp synthase (7 kDa, Complex from Bos taurus)
- Atp synthase subunit atp5mpl, mitochondrial (6 kDa, Protein from cattle)
- Cardiolipin (1 kDa, Ligand)
- Atp synthase subunit f, mitochondrial (10 kDa, Protein from cattle)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Atp synthase membrane subunit dapit, mitochondrial (6 kDa, Protein from cattle)
- Atp synthase subunit d, mitochondrial (18 kDa, Protein from cattle)
bovine ATP synthase stator domain, state 1
Resolution: 4.33 Å
EM Method: Single-particle
Fitted PDBs: 6ziq
Q-score: 0.385
Spikes TE, Montgomery MG, Walker JE
PNAS (2020) 117 pp. 23519-23526 [ Pubmed: 32900941 DOI: doi:10.1073/pnas.2013998117 ]
- Atp synthase f(0) complex subunit c1, mitochondrial (7 kDa, Protein from Bovine)
- Atp synthase membrane subunit dapit, mitochondrial (6 kDa, Protein from Bovine)
- Atp synthase stator domain, state 1 (Complex from Bos taurus)
- Atp synthase subunit a (24 kDa, Protein from Bovine)
- Atp synthase peripheral stalk (Complex from Bos taurus)
- Atp synthase subunit atp5mpl, mitochondrial (6 kDa, Protein from Bovine)
- Atp synthase subunit g, mitochondrial (11 kDa, Protein from Bovine)
- Atp synthase subunit e, mitochondrial (8 kDa, Protein from Bovine)
- Atp synthase f(0) complex subunit b1, mitochondrial (24 kDa, Protein from Bovine)
- Atp synthase subunit f, mitochondrial (10 kDa, Protein from Bovine)
- Atp synthase protein 8 (7 kDa, Protein from Bovine)
- Atp synthase-coupling factor 6, mitochondrial (8 kDa, Protein from Bovine)
- Atp synthase subunit d, mitochondrial (18 kDa, Protein from Bovine)
- Atp synthase subunit o, mitochondrial (20 kDa, Protein from Bovine)
- Bovine atp synthase (73 kDa, Complex)
- Atp synthase subunit alpha, mitochondrial (55 kDa, Protein from Bovine)
bovine ATP synthase Stator domain, state 2
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 6zit
Q-score: 0.371
Spikes TE, Montgomery MG, Walker JE
PNAS (2020) 117 pp. 23519-23526 [ Pubmed: 32900941 DOI: doi:10.1073/pnas.2013998117 ]
- Atp synthase membrane subunit dapit, mitochondrial (6 kDa, Protein from Bovine)
- Atp synthase subunit a (24 kDa, Protein from Bovine)
- Atp synthase peripheral stalk (Complex from Bos taurus)
- Atp synthase subunit atp5mpl, mitochondrial (6 kDa, Protein from Bovine)
- Atp synthase subunit g, mitochondrial (11 kDa, Protein from Bovine)
- Atp synthase subunit e, mitochondrial (8 kDa, Protein from Bovine)
- Atp synthase f(0) complex subunit b1, mitochondrial (24 kDa, Protein from Bovine)
- Atp synthase subunit f, mitochondrial (10 kDa, Protein from Bovine)
- Atp synthase protein 8 (7 kDa, Protein from Bovine)
- Atp synthase-coupling factor 6, mitochondrial (8 kDa, Protein from Bovine)
- Atp synthase stator domain, state 2 (Complex from Bos taurus)
- Bovine dimeric atp synthase (7 kDa, Complex)
- Atp synthase f(0) complex subunit c2, mitochondrial (7 kDa, Protein from Bovine)
- Atp synthase subunit o, mitochondrial (20 kDa, Protein from Bovine)
- Atp synthase subunit d, mitochondrial (18 kDa, Protein from Bovine)
- Atp synthase subunit alpha, mitochondrial (55 kDa, Protein from Bovine)
bovine ATP synthase monomer state 1 (combined)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6zpo
Q-score: 0.529
Spikes TE, Montgomery MG, Walker JE
PNAS (2020) 117 pp. 23519-23526 [ Pubmed: 32900941 DOI: doi:10.1073/pnas.2013998117 ]
- Atp synthase subunit a (24 kDa, Protein from Bovine)
- Atp synthase subunit e, mitochondrial (8 kDa, Protein from Bovine)
- Atp synthase subunit g, mitochondrial (11 kDa, Protein from Bos taurus)
- Atp synthase subunit d, mitochondrial (18 kDa, Protein from Bovine)
- Atp synthase subunit alpha, mitochondrial (55 kDa, Protein from Bovine)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Bovine atp synthase (Complex from Bos taurus)
- Bovine atp synthase (Complex from synthetic construct)
- Atp synthase subunit f, mitochondrial (10 kDa, Protein from Bovine)
- Atp synthase protein 8 (7 kDa, Protein from Bovine)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Water (18 Da, Ligand)
- Atp synthase f(0) complex subunit c1, mitochondrial (7 kDa, Protein from Bovine)
- Cardiolipin (1 kDa, Ligand)
- Atp synthase subunit atp5mpl, mitochondrial (6 kDa, Protein from Bos taurus)
- Atpase inhibitor, mitochondrial (7 kDa, Protein from Bos taurus)
- Atp synthase f(0) complex subunit b1, mitochondrial (24 kDa, Protein from Bovine)
- Magnesium ion (24 Da, Ligand)
- Atp synthase membrane subunit dapit, mitochondrial (6 kDa, Protein from Bos taurus)
- Atp synthase subunit delta, mitochondrial (15 kDa, Protein from Bovine)
- Atp synthase subunit o, mitochondrial (20 kDa, Protein from Bovine)
- Atp synthase subunit epsilon, mitochondrial (5 kDa, Protein from Bovine)
- Atp synthase subunit beta, mitochondrial (51 kDa, Protein from Bovine)
- Bovine atp synthase (1 MDa, Complex)
- Atp synthase-coupling factor 6, mitochondrial (8 kDa, Protein from Bos taurus)
- Atp synthase subunit gamma, mitochondrial (30 kDa, Protein from Bos taurus)
- Monomeric bovine atp synthase, state 1 (Complex from Bos taurus)