The V1 region of bovine V-ATPase in complex with human mEAK7 (focused refinement)
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 7une
Q-score: 0.496
Nat Commun (2022) 13 pp. 3272-3272 [ DOI: doi:10.1038/s41467-022-30899-z Pubmed: 35672408 ]
- V-type proton atpase subunit b, brain isoform (56 kDa, Protein from Bos taurus)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Bos taurus)
- V-type proton atpase subunit g (13 kDa, Protein from Bos taurus)
- V-type proton atpase subunit d (28 kDa, Protein from Bos taurus)
- Kiaa1609 protein, isoform cra_a (51 kDa, Protein from Homo sapiens)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Bos taurus)
- Cryo-em structure of the v1 region of bovine v-atpase in complex with human meak7 (Complex from Bos taurus)
CryoEM structure of a mEAK7 bound human V-ATPase complex
Resolution: 4.08 Å
EM Method: Single-particle
Fitted PDBs: 7unf
Q-score: 0.479
Nat Commun (2022) 13 pp. 3272-3272 [ DOI: doi:10.1038/s41467-022-30899-z Pubmed: 35672408 ]
- V-type proton atpase subunit d (28 kDa, Protein from Homo sapiens)
- V-type proton atpase subunit g 1 (13 kDa, Protein from Homo sapiens)
- Kiaa1609 protein, isoform cra_a (51 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- V-type proton atpase subunit e 1 (9 kDa, Protein from Homo sapiens)
- V-type proton atpase subunit s1 (52 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ribonuclease kappa (15 kDa, Protein from Homo sapiens)
- Atpase h(+)-transporting lysosomal accessory protein 2 (38 kDa, Protein from Homo sapiens)
- V-type proton atpase subunit h (55 kDa, Protein from Homo sapiens)
- V-type proton atpase 21 kda proteolipid subunit (21 kDa, Protein from Homo sapiens)
- V-type proton atpase 16 kda proteolipid subunit (15 kDa, Protein from Homo sapiens)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Homo sapiens)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Homo sapiens)
- Cryoem structure of meak7 bound human v-atpase complex (Complex from Homo sapiens)
- V-type proton atpase 116 kda subunit a 4 (98 kDa, Protein from Homo sapiens)
- V-type proton atpase subunit b, brain isoform (56 kDa, Protein from Homo sapiens)
- V-type proton atpase subunit d 1 (40 kDa, Protein from Homo sapiens)
- V-type proton atpase subunit c 1 (43 kDa, Protein from Homo sapiens)
- V-type proton atpase subunit f (13 kDa, Protein from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Cryo-EM structure of bafilomycin A1-bound intact V-ATPase from bovine brain
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 7khr
Q-score: 0.493
Wang R, Wang J, Hassan A, Lee CH, Xie XS, Li X
Nat Commun (2021) 12 pp. 1782-1782 [ Pubmed: 33741963 DOI: doi:10.1038/s41467-021-22111-5 ]
- V-type proton atpase subunit e 2 (9 kDa, Protein from Bos taurus)
- Renin receptor (39 kDa, Protein from Bos taurus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- V-type proton atpase 16 kda proteolipid subunit (15 kDa, Protein from Bos taurus)
- V-type proton atpase subunit a (96 kDa, Protein from Bos taurus)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- V-type proton atpase 21 kda proteolipid subunit (21 kDa, Protein from Bos taurus)
- (5r)-2,4-dideoxy-1-c-{(2s,3r,4s)-3-hydroxy-4-[(2r,3s,4e,6e,9r,10s,11r,12e,14z)-10-hydroxy-3,15-dimethoxy-7,9,11,13-tetramethyl-16-oxo-1-oxacyclohexadeca-4,6,12,14-tetraen-2-yl]pentan-2-yl}-4-methyl-5-propan-2-yl-alpha-d-threo-pentopyranose (622 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- V-type proton atpase subunit g (13 kDa, Protein from Bos taurus)
- V-type proton atpase subunit d (28 kDa, Protein from Bos taurus)
- Ribonuclease kappa (11 kDa, Protein from Bos taurus)
- V-type proton atpase subunit b, brain isoform (56 kDa, Protein from Bos taurus)
- Cryo-em structure of bafilomycin a1-bound intact v-atpase from bovine brain (Complex from Bos taurus)
- V-type proton atpase subunit f (13 kDa, Protein from Bos taurus)
- Methyl (3r,6z,10e,14e)-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraen-1-yl dihydrogen diphosphate (534 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Bos taurus)
- Oleic acid (282 Da, Ligand)
- V-type proton atpase subunit c 1 (44 kDa, Protein from Bos taurus)
- V-type proton atpase subunit s1 (51 kDa, Protein from Bos taurus)
- V-type proton atpase subunit h (54 kDa, Protein from Bos taurus)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Bos taurus)
- V-type proton atpase subunit d 1 (40 kDa, Protein from Bos taurus)
Cryo-EM structure of V-ATPase from bovine brain, state 1
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 6xbw
Q-score: 0.479
Wang R, Long T, Hassan A, Wang J, Sun Y, Xie XS, Li X
Nat Commun (2020) 11 pp. 3921-3921 [ Pubmed: 32764564 DOI: doi:10.1038/s41467-020-17762-9 ]
- V-type proton atpase subunit e 2 (9 kDa, Protein from Bos taurus)
- Renin receptor (39 kDa, Protein from Bos taurus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- V-type proton atpase 16 kda proteolipid subunit (15 kDa, Protein from Bos taurus)
- V-type proton atpase subunit a (96 kDa, Protein from Bos taurus)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- V-type proton atpase 21 kda proteolipid subunit (21 kDa, Protein from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- V-type proton atpase subunit g (13 kDa, Protein from Bos taurus)
- V-type proton atpase subunit d (28 kDa, Protein from Bos taurus)
- Ribonuclease kappa (11 kDa, Protein from Bos taurus)
- V-type proton atpase subunit b, brain isoform (56 kDa, Protein from Bos taurus)
- V-type proton atpase subunit f (13 kDa, Protein from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Bos taurus)
- Oleic acid (282 Da, Ligand)
- V-type proton atpase subunit c 1 (44 kDa, Protein from Bos taurus)
- V-type proton atpase subunit s1 (51 kDa, Protein from Bos taurus)
- V-type proton atpase subunit h (54 kDa, Protein from Bos taurus)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Bos taurus)
- Cryo-em structure of v-atpase complex from bovine brain, state 1 (Complex from Bos taurus)
- V-type proton atpase subunit d 1 (40 kDa, Protein from Bos taurus)
Cryo-EM structure of V-ATPase from bovine brain, state 2
Resolution: 3.79 Å
EM Method: Single-particle
Fitted PDBs: 6xby
Q-score: 0.432
Wang R, Long T, Hassan A, Wang J, Sun Y, Xie XS, Li X
Nat Commun (2020) 11 pp. 3921-3921 [ Pubmed: 32764564 DOI: doi:10.1038/s41467-020-17762-9 ]
- Renin receptor (39 kDa, Protein from Bos taurus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- V-type proton atpase 16 kda proteolipid subunit (15 kDa, Protein from Bos taurus)
- V-type proton atpase subunit a (96 kDa, Protein from Bos taurus)
- V-type proton atpase 21 kda proteolipid subunit (21 kDa, Protein from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- V-type proton atpase subunit g (13 kDa, Protein from Bos taurus)
- V-type proton atpase subunit d (28 kDa, Protein from Bos taurus)
- V-type proton atpase subunit b, brain isoform (56 kDa, Protein from Bos taurus)
- V-type proton atpase subunit f (13 kDa, Protein from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Bos taurus)
- V-type proton atpase subunit d 1 (40 kDa, Protein from Bos taurus)
- Cryo-em structure of v-atpase complex from bovine brain, state 2 (Complex from Bos taurus)
- V-type proton atpase subunit c 1 (44 kDa, Protein from Bos taurus)
- V-type proton atpase subunit h (54 kDa, Protein from Bos taurus)
- V-type proton atpase subunit s1 (51 kDa, Protein from Bos taurus)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Bos taurus)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)