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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"uL5" AND resolution:[2 TO 3} AND software_ft:"CRYOSPARC" AND author_name:"M"

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EMD-51843

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mature 50S subunit

Release date: Jan. 22, 2025
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 9h3z
Q-score: 0.394

Lauer SM, Gasse J, Krizsan A, Reepmeyer M, Sprink T, Nikolay R, Spahn CMT, Hoffmann R

Nat Commun (2025) 16 pp. 567-567 [ Pubmed: 39794318 DOI: doi:10.1038/s41467-025-55836-8 ]

Sample:

50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
Pre50s precursor derived from api137-treated cells (Complex from Escherichia coli)
Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
23s ribosomal rrna (941 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)

[Show more]

EMD-18458

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E167K RF2 on E. coli 70S release complex with UAA

Release date: Sept. 25, 2024
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8qk7
Q-score: 0.568

Pundir S, Larsson DSD, Selmer M, Sanyal S

To Be Published

Sample:

Small ribosomal subunit protein us7 (20 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein bs16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
Peptide chain release factor rf2 (41 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein bs6, fully modified isoform (15 kDa, Protein from Escherichia coli)
Potassium ion (39 Da, Ligand)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
1,4-diaminobutane (88 Da, Ligand)
Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
Mrna (15 kDa, RNA from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
Spermine (202 Da, Ligand)
Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
Ribosome release complex (2 MDa, Complex from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
Spermidine (145 Da, Ligand)
Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
23s rrna (941 kDa, RNA from Escherichia coli)
Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
Trna(fmet) (24 kDa, RNA from Escherichia coli)
Small ribosomal subunit protein bs21 (8 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
16s rrna (499 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul11 (14 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
5s rrna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)

[Show more]

EMD-19426

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Escherichia coli 50S subunit in complex with the antimicrobial peptide Api137

Release date: May 22, 2024
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8rpy
Q-score: 0.519

Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubmuller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R

Nat Commun (2024) 15 pp. 3945-3945 [ Pubmed: 38730238 DOI: doi:10.1038/s41467-024-48027-4 ]

Sample:

Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
Api137 (Complex from Apis mellifera)
Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
Apidaecins type 137 (2 kDa, Protein from Apis mellifera)
Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
Api137 in complex with the large ribosomal subunit (Complex)
Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
Large ribosomal subunit (Complex from Escherichia coli)
Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)

[Show more]

EMD-19427

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Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation I

Release date: May 22, 2024
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 8rpz
Q-score: 0.557

Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubmuller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R

Nat Commun (2024) 15 pp. 3945-3945 [ Pubmed: 38730238 DOI: doi:10.1038/s41467-024-48027-4 ]

Sample:

Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
Api88 in complex with the large ribosomal subunit - conformation i (Complex)
Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
Api88 (Complex from Apis mellifera)
Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
Apidaecins type 88 (2 kDa, Protein from Apis mellifera)
Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
Large ribosomal subunit (Complex from Escherichia coli)
Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)

[Show more]

EMD-19428

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Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation II

Release date: May 22, 2024
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 8rq0
Q-score: 0.557

Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubmuller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R

Nat Commun (2024) 15 pp. 3945-3945 [ Pubmed: 38730238 DOI: doi:10.1038/s41467-024-48027-4 ]

Sample:

Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
Api88 in complex with the large ribosomal subunit - conformation ii (Complex)
Api88 (Complex from Apis mellifera)
Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
Apidaecins type 88 (2 kDa, Protein from Apis mellifera)
Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
Large ribosomal subunit (Complex from Escherichia coli)
Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)

[Show more]

EMD-19429

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Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation III

Release date: May 22, 2024
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 8rq2

Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubmuller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R

Nat Commun (2024) 15 pp. 3945-3945 [ Pubmed: 38730238 DOI: doi:10.1038/s41467-024-48027-4 ]

Sample:

Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
Api88 in complex with the large ribosomal subunit - conformation iii (Complex)
Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
Api88 (Complex from Apis mellifera)
Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
Apidaecins type 88 (2 kDa, Protein from Apis mellifera)
Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
Large ribosomal subunit (Complex from Escherichia coli)
Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)

[Show more]

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EMDB [6]

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