EMPIAR-10104 FIB-SEM of a dividing cell at 5.3 min after anaphase onset

FIB-SEM of a dividing cell at 5.3 min after anaphase onset

Entry authors:

Otsuka S , Steyer AM , Schorb M , Hériché JK , Hossain MJ , Sethi S, Kueblbeck M, Schwab Y, Beck M , Ellenberg J

Publication:

Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings

Otsuka S , Steyer AM , Schorb M , Hériché JK , Hossain MJ , Sethi S, Kueblbeck M, Schwab Y, Beck M , Ellenberg J

Nat Struct Mol Biol 25 21-28 (2017)

PMID: 29323269

DOI: 10.1038/s41594-017-0001-9

Experiment type:

FIB-SEM

Deposited:

2017-05-24

Released:

2017-11-30

Last modified:

2021-10-25

Dataset size:

31.2 GB

Dataset DOI:

10.6019/EMPIAR-10104

Experimental metadata:

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Contains:

micrographs

Image set

1. FIB-SEM images of a dividing cell at 5.3 min after anaphase onset

Category:micrographs - multiframe

Image format:TIFF

No. of images or tilt series:1998

Frames per image:1

Image size:(4892, 3427)

Pixel type:UNSIGNED BYTE

Pixel spacing:(5 Å, 8 Å)

Details:

The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm. The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). We used the second half of the cell for segmentation.

data 31.2 GB

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10104 31.2 GB

EMPIAR-10104

FIB-SEM of a dividing cell at 5.3 min after anaphase onset

Image set

1. FIB-SEM images of a dividing cell at 5.3 min after anaphase onset

Browse All Files

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