EMPIAR-10104

FIB-SEM of a dividing cell at 5.3 min after anaphase onset

Entry authors:
Otsuka S , Steyer AM , Schorb M , Hériché JK , Hossain MJ , Sethi S, Kueblbeck M, Schwab Y, Beck M , Ellenberg J 
Publication:
Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings
Otsuka S , Steyer AM , Schorb M , Hériché JK , Hossain MJ , Sethi S, Kueblbeck M, Schwab Y, Beck M , Ellenberg J 
Nat Struct Mol Biol 25 21-28 (2017)
PMID: 29323269
Experiment type:
FIB-SEM
Deposited:
2017-05-24
Released:
2017-11-30
Last modified:
2021-10-25
Dataset size:
31.2 GB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. FIB-SEM images of a dividing cell at 5.3 min after anaphase onset
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:1998
Frames per image:1
Image size:(4892, 3427)
Pixel type:UNSIGNED BYTE
Pixel spacing:(5 Å, 8 Å)
Details:
.The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm. The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). We used the second half of the cell for segmentation.
XAvailable download options:
Loading...

Browse All Files

empair
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.