EMPIAR-10141

CryoET of apoferritin with 0.5 mM TCEP single particle

Entry authors:
Noble AJ , Dandey VP, Wei H, Brasch J, Chase J, Acharya P, Tan YZ , Zhang Z, Kim LY, Scapin G, Rapp M, Eng ET , Rice MJ, Cheng A, Negro CJ, Shapiro L, Kwong PD, Jeruzalmi D, des Georges A, Potter CS, Carragher B 
Publication:
Routine single particle CryoEM sample and grid characterization by tomography
Noble AJ , Dandey VP, Wei H, Brasch J, Chase J, Acharya P, Tan YZ , Zhang Z, Kim LY, Scapin G, Rapp M, Eng ET , Rice WJ, Cheng A, Negro CJ, Shapiro L, Kwong PD, Jeruzalmi D , des Georges A, Potter CS , Carragher B 
Elife 7 (2018)
PMID: 29809143
Experiment type:
EMDB
Related EMDB entry:
Deposited:
2017-12-06
Released:
2018-04-05
Last modified:
2022-07-21
Dataset size:
35.3 GB
Version history:
1
2018-04-05
The depositor added tomography particle picks.
Experimental metadata:
Contains:
tilt series
picked particles
Image not available

Image sets

1. Tilt-series image stacks
Category:tilt series
Image format:MRC
No. of images or tilt series:3
Image size:(3712, 4984)
Pixel type:SIGNED 16 BIT INTEGER
Pixel spacing:(2.34 Å, 2.34 Å)
Details:
.Not frame-aligned.
.mdoc files included.
2. Appion-Protomo tilt-series alignments
Category:tilt series
Image format:MRC
No. of images or tilt series:3
Image size:(3712, 3838)
Pixel type:32 BIT FLOAT
Pixel spacing:(2.34 Å, 2.34 Å)
Details:
.Includes a full tilt-series alignment run for each tilt-series. To view properly, navigate to the protomo_alignments folder in Appion-Protomo.
3. Manual particle picking in Dynamo
Category:picked particles - single frame - processed
Image format:MRC
No. of images or tilt series:1
Pixel type:32 BIT FLOAT
Details:
.Binned by 4 manual particle picking of the tomogram using Dynamo in TBL and OMD formats that relate to the corresponding .mrc file. Manual picking was performed to accurately distinguish between particles adsorbed to the air-water interfaces, non-adsorbed particles, and particles over or adsorbed to carbon.
XAvailable download options:
Loading...

Browse All Files

empair
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.