EMPIAR-10490

Integrative imaging reveals SARS-CoV-2 induced reshaping of subcellular morphology

Entry authors:
Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R
Publication:
Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies
Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R
Cell Host Microbe (2020)
PMID: 33245857
Experiment type:
IHM
Related EMDB entry:
Deposited:
2020-06-24
Released:
2020-12-11
Last modified:
2021-10-25
Dataset size:
1.1 TB
Dataset DOI:
10.6019/EMPIAR-10490
Experimental metadata:
Download xml
Contains:
tilt series
reconstructed volumes
micrographs
Image not available

Image sets

1. MOI0.5-24h-Dual axis tilt series
Category:tilt series
Image format:MRC
No. of images or tilt series:202
Image size:(2024, 2024)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: MOI = 0.5, 24h post infection;
Dual axis tilt series, both axes as individual *.st MRC files
2. MOI5-12h-Dual axis tilt series
Category:tilt series
Image format:MRC
No. of images or tilt series:178
Image size:(2024, 2024)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: MOI = 5, 12h post infection;
Dual axis tilt series, both axes as individual *.st MRC files
3. MOI5-24h-Dual axis tilt series
Category:tilt series
Image format:MRC
No. of images or tilt series:152
Image size:(2024, 2024)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: MOI = 5, 24h post infection;
Dual axis tilt series, both axes as individual *.st MRC files
4. MOI5-6h-Dual axis tilt series
Category:tilt series
Image format:MRC
No. of images or tilt series:14
Image size:(2024, 2024)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: MOI = 5, 6h post infection;
Dual axis tilt series, both axes as individual *.st MRC files
5. MOI0.5-24h-Reconstructed tomograms
Category:reconstructed volumes
Image format:BIG DATA VIEWER HDF5
No. of images or tilt series:97
Frames per image:1
Pixel type:UNSIGNED BYTE
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: MOI = 0.5, 24h post infection;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
6. MOI5-12h-Reconstructed tomograms
Category:reconstructed volumes
Image format:BIG DATA VIEWER HDF5
No. of images or tilt series:85
Frames per image:1
Pixel type:UNSIGNED BYTE
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: MOI = 5, 12h post infection;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
7. MOI5-24h-Reconstructed tomograms
Category:reconstructed volumes
Image format:BIG DATA VIEWER HDF5
No. of images or tilt series:56
Frames per image:1
Pixel type:UNSIGNED BYTE
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: MOI = 5, 24h post infection;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
8. MOI5-6h-Reconstructed tomograms
Category:reconstructed volumes
Image format:BIG DATA VIEWER HDF5
No. of images or tilt series:7
Frames per image:1
Pixel type:UNSIGNED BYTE
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: MOI = 5, 6h post infection;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
9. S4 Area 2 FIB-SEM Volume 8nm isotropic
Category:micrographs - single frame
Image format:TIFF
No. of images or tilt series:4824
Image size:(3864, 3144)
Pixel type:UNSIGNED BYTE
Pixel spacing:(80 Å, 80 Å)
Details:
.S4 Area 2 FIB-SEM Volume 8nm isotropic voxel size, aligned
10. S4 Area 3 FIB-SEM Volume 8nm isotropic
Category:micrographs - single frame
Image format:TIFF
No. of images or tilt series:4413
Image size:(5632, 3648)
Pixel type:UNSIGNED BYTE
Pixel spacing:(80 Å, 80 Å)
Details:
.S4 Area 3 FIB-SEM Volume 8nm isotropic voxel size, aligned
11. S4 Cell 1 FIB-SEM Volume 5nm isotropic
Category:micrographs - single frame
Image format:TIFF
No. of images or tilt series:11199
Image size:(7512, 3864)
Pixel type:UNSIGNED BYTE
Pixel spacing:(50 Å, 50 Å)
Details:
.S4 Cell 1 FIB-SEM Volume 5nm isotropic voxel size, aligned
12. S5 Mock Cell 1 FIB-SEM Volume 8nm isotropic
Category:micrographs - single frame
Image format:TIFF
No. of images or tilt series:2574
Image size:(6624, 4236)
Pixel type:UNSIGNED BYTE
Pixel spacing:(80 Å, 80 Å)
Details:
.S5 Mock/Control Cell 1 FIB-SEM Volume 8nm isotropic voxel size, aligned
13. Mock cells - Dual axis tilt series
Category:tilt series
Image format:MRC
No. of images or tilt series:26
Image size:(2024, 2024)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: Mock;
Dual axis tilt series, both axes as individual *.st MRC files
14. Mock cells-Reconstructed tomograms
Category:reconstructed volumes
Image format:BIG DATA VIEWER HDF5
No. of images or tilt series:13
Frames per image:1
Pixel type:UNSIGNED BYTE
Pixel spacing:(15.578 Å, 15.578 Å)
Details:
.Condition: Mock;
Reconstructed volume from dual axis tilt series, BDV HDF5 format, positioned according to physical location on specimen. Corresponding map files for targeting in adjacent "maps" directory.
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