EMPIAR-10718 The structure of human CST reveals a decameric assembly bound to telomeric DNA

The structure of human CST reveals a decameric assembly bound to telomeric DNA

Entry authors:

Lim C , Barbour AT , Zaug AJ , Goodrich KJ , McKay AE , Wuttke DS , Cech TR

Publication:

The structure of human CST reveals a decameric assembly bound to telomeric DNA

Lim C , Barbour AT , Zaug AJ , Goodrich KJ , McKay AE , Wuttke DS , Cech TR

Science 368 1081-1085 (2020)

PMID: 32499435

DOI: 10.1126/science.aaz9649

Experiment type:

EMDB

Related PDB entry:

6w6w

Related EMDB entry:

EMD-21567 (3.0Å)

Deposited:

2021-05-17

Released:

2021-06-25

Last modified:

2021-06-25

Dataset size:

14.3 TB

Dataset DOI:

10.6019/EMPIAR-10718

Experimental metadata:

Download xml

Contains:

micrographs

Image sets

1. Unaligned movies of CST with 3xTEL DNA oligomer at 0 degrees stage tilt

Category:micrographs - multiframe

Image format:TIFF

No. of images or tilt series:20808

Frames per image:60

Image size:(11520, 8184)

Pixel type:UNSIGNED BYTE

Details:

This dataset was collected on a KRIOS with 3x3 beam tilt strategy using SerialEM. The optical groups are named just before the date. For example, W3_3_999_009_Aug02_00.49.32.tif is group 9. In addition, this dataset was collected at 0 degrees stage tilt with a pixel size of 0.539 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (60 frames) using a Gatan K3 direct electron detector in super-resolution CDS mode. Gain reference to be applied.

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2. Unaligned movies of CST with 3xTEL DNA oligomer at 30 degrees stage tilt

Category:micrographs - multiframe

Image format:TIFF

No. of images or tilt series:7227

Frames per image:60

Image size:(11520, 8184)

Pixel type:UNSIGNED BYTE

Details:

This dataset was collected on a KRIOS with 3x3 beam tilt strategy using SerialEM. The optical groups are named just before the date. For example, W3_3_30tilt_3315_004_Aug08_10.16.04.tif is group 4. In addition, this dataset was collected at 30 degrees stage tilt with a pixel size of 0.539 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (60 frames) using a Gatan K3 direct electron detector in super-resolution CDS mode. Gain reference to be applied.