EMPIAR-10907 FIB-SEM of an Mbp-deficient shiverer mouse optic nerve

FIB-SEM of an Mbp-deficient shiverer mouse optic nerve

Entry authors:

Meschkat M, Steyer AM , Ruhwedel T , Möbius W

Publication:

White matter integrity in mice requires continuous myelin synthesis at the inner tongue

Meschkat M, Steyer AM , Weil MT, Kusch K , Jahn O, Piepkorn L, Agüi-Gonzalez P , Phan NTN, Ruhwedel T , Sadowski B, Rizzoli SO , Werner HB , Ehrenreich H , Nave KA , Möbius W

Nat Commun 13 (2022)

PMID: 35246535

DOI: 10.1038/s41467-022-28720-y

Experiment type:

FIB-SEM

Deposited:

2021-12-17

Released:

2022-01-12

Last modified:

2022-10-17

Dataset size:

18.8 GB

Dataset DOI:

10.6019/EMPIAR-10907

Experimental metadata:

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Contains:

micrographs

Image set

1. FIB-SEM data stack of an Mbp-deficient shiverer mouse optic nerve

Category:micrographs - single frame

Image format:TIFF

No. of images or tilt series:857

Image size:(4650, 5076)

Pixel type:UNSIGNED BYTE

Pixel spacing:(50 Å, 50 Å)

Details:

Optic nerve of a male Mbp-deficient mouse (shiverer), perfusion fixation: 2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl, rOTO embedding, Durcupan.
FIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 700 pA, z-step 50 nm
Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ

FIB-SEM Data set 20171117_shiverer rOTO 18.8 GB

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EMPIAR-10907

FIB-SEM of an Mbp-deficient shiverer mouse optic nerve

Image set

1. FIB-SEM data stack of an Mbp-deficient shiverer mouse optic nerve

Browse All Files

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