EMPIAR-10907

FIB-SEM of an Mbp-deficient shiverer mouse optic nerve

Entry authors:
Meschkat M, Steyer AM , Ruhwedel T , Möbius W 
Publication:
White matter integrity in mice requires continuous myelin synthesis at the inner tongue
Meschkat M, Steyer AM , Weil MT, Kusch K , Jahn O, Piepkorn L, Agüi-Gonzalez P , Phan NTN, Ruhwedel T , Sadowski B, Rizzoli SO , Werner HB , Ehrenreich H , Nave KA , Möbius W 
Nat Commun 13 (2022)
PMID: 35246535
Experiment type:
FIB-SEM
Deposited:
2021-12-17
Released:
2022-01-12
Last modified:
2022-10-17
Dataset size:
18.8 GB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. FIB-SEM data stack of an Mbp-deficient shiverer mouse optic nerve
Category:micrographs - single frame
Image format:TIFF
No. of images or tilt series:857
Image size:(4650, 5076)
Pixel type:UNSIGNED BYTE
Pixel spacing:(50 Å, 50 Å)
Details:
.Optic nerve of a male Mbp-deficient mouse (shiverer), perfusion fixation: 2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl, rOTO embedding, Durcupan.
FIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 700 pA, z-step 50 nm
Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ
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