EMPIAR-10914

Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and delta subunits, in presence of GABA, histamine and nanobody Nb25

Entry authors:
Sente A , Desai R, Naydenova K , Malinauskas T , Jounaidi Y, Miehling J, Zhou X, Masiulis S , Hardwick SW , Chirgadze DY , Miller KW , Aricescu AR 
Publication:
Differential assembly diversifies GABAA receptor structures and signalling
Aricescu AR , Sente A , Desai R, Naydenova K , Malinauskas T , Jounaidi Y, Miehling J, Zhou X, Masiulis S , Hardwick SW , Chirgadze DY , Miller KW 
Nature (2022)
PMID: 35355020
Experiment type:
EMDB
Related PDB entries:
Related EMDB entries:
.EMD-14070 (3.1Å), EMD-14069 (3.0Å)
Deposited:
2021-12-29
Released:
2022-04-04
Last modified:
2022-04-04
Dataset size:
3.0 TB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Unaligned multi-frame micrographs of GABA(A) receptors purified from cells expressing human full-length alpha4, beta3 and delta subunits, in presence of GABA, histamine and nanobody Nb25
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:11562
Frames per image:48
Image size:(11520, 8184)
Pixel type:UNSIGNED BYTE
Pixel spacing:(0.326 Å, 0.326 Å)
Details:
.K3 super-resolution movies with a total dose of 47.15 e/A2 and exposure time of 1.31 s.
The gain reference used for data processing is given in data/superres-gain.mrc. No rotation or flipping is required.
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