EMPIAR-11140

Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the cingulate cortex of an individual with Parkinson's disease dementia -synuclein filaments

Entry authors:
Yang Y , Shi Y , Schweighauser M , Zhang X, Kotecha A , Murzin AG, Garringer HJ , Cullinane PW, Saito Y, Foroud T , Warner TT , Hasegawa K, Vidal R, Murayama S, Revesz T, Ghetti B , Hasegawa M , Lashley T , Scheres SHW , Goedert M 
Publication:
Structures of α-synuclein filaments from human brains with Lewy pathology
Yang Y , Shi Y , Schweighauser M , Zhang X, Kotecha A , Murzin AG, Garringer HJ , Cullinane PW, Saito Y, Foroud T , Warner TT , Hasegawa K, Vidal R, Murayama S, Revesz T, Ghetti B , Hasegawa M , Lashley T , Scheres SHW , Goedert M 
Nature 610 791-795 (2022)
PMID: 36108674
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-15285 (2.2Å)
Deposited:
2022-06-28
Released:
2022-11-11
Last modified:
2022-11-11
Dataset size:
1.2 TB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Cryo-EM reconstruction of alpha-synuclein filaments from Parkinson's disease dementia
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:10874
Frames per image:42
Image size:(4096, 4096)
Pixel type:UNSIGNED BYTE
Pixel spacing:(0.727 Å, 0.727 Å)
Details:
.The files were written as eer files by EPU and then converted to TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 0.727 A/px. The dose rate is 1 e/A2/frame for Dataset1 and Dataset2. Please use gain-reference accordingly without any rotation or flipping.
picking contains start-end coordinates of the manually picked filaments of Dataset1 and Dataset2, respectively.
Refine070_run_data.star contains STAR file(s) with the metadata of the final refined segments.
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