EMPIAR-11283

LRRC8A-BRIL:C Heteromer in GDN

Entry authors:
Publication:
Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels
Kern DM , Bleier J , Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY , Brohawn SG 
bioRxiv (2022)
PMID: 30775971
Experiment type:
EMDB
Related PDB entries:
Related EMDB entries:
.EMD-27681 (4.16Å), EMD-27679 (4.06Å), EMD-27678 (4.12Å), EMD-27682 (3.07Å), EMD-27675 (3.98Å), EMD-27674 (3.04Å), EMD-27676 (3.18Å), EMD-27677 (2.95Å)
Deposited:
2022-08-02
Released:
2023-04-12
Last modified:
2023-04-13
Dataset size:
8.9 TB
Dataset DOI:
10.6019/EMPIAR-11283
Experimental metadata:
Download xml
Contains:
micrographs
picked particles
Image not available

Image sets

1. Unaligned tif movies for LRRC8ABRIL:C in 150 mM KCl
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:8261
Frames per image:50
Image size:(11520, 8184)
Pixel type:UNSIGNED BYTE
Pixel spacing:(0.524 Å, 0.524 Å)
Details:
.Gain reference in dm4 and mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 1.076 e/Å2 dose per frame and use bin 2 to produce mrc at the physical pixel size of 1.048Å/px.
2. Unaligned tif movies for LRRC8ABRIL:C in 75 mM KCl
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:7208
Frames per image:50
Image size:(11520, 8184)
Pixel type:UNSIGNED BYTE
Pixel spacing:(0.524 Å, 0.524 Å)
Details:
.Gain reference in dm4 and mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 0.9953 e/Å2 dose per frame and use bin 2 to produce mrc at the physical pixel size of 1.048Å/px.
3. Pooled polished particles stacks for LRRC8A-BRIL/C in GDN
Category:picked particles - multiframe - processed
Image format:MRCS
No. of images or tilt series:11609
Frames per image:1
Image size:(416, 416)
Pixel type:UNSIGNED BYTE
Pixel spacing:(1.048 Å, 1.048 Å)
Details:
.Contains polished particles (437,174) as 11,609 mrcs files from the pooled GDN samples (75 and 150 mM KCl). The selections of particles for each conformation (1 and 2) are in the main data folder. They are in RELION star format and can be imported into cryoSPARC as well for processing.
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