EMPIAR-11349

Cryo-EM structure of a delivery complex containing the SspB adaptor, an ssrA-tagged substrate, and the AAA+ ClpXP protease

Entry authors:
Publication:
The SspB adaptor drives structural changes in the AAA+ClpXP protease during ssrA-tagged substrate delivery
bioRxiv (2022)
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-28585 (3.7Å)
Deposited:
2022-11-30
Released:
2023-01-31
Last modified:
2023-01-31
Dataset size:
4.0 TB
Dataset DOI:
10.6019/EMPIAR-11349
Experimental metadata:
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Contains:
micrographs
Image not available

Image set

1. Unaligned Movies of a Delivery Complex Containing the SspB Adaptor, an ssrA-Tagged Substrate, and the AAA+ ClpXP Protease
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:9524
Frames per image:40
Image size:(11520, 8184)
Pixel type:UNSIGNED BYTE
Pixel spacing:(0.435 Å, 0.435 Å)
Details:
.Nominal Defocus range (min, max, step): -1.0, -2.5, -0.25
Total exposure on specimen: 75.98 el/A2
Exposure time: 2.02 s
Nominal pixel size: 0.87 A (0.435 A super res)
Calibrated pixel size: 0.832 (0.416 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3
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