EMPIAR-11364

Cryogenic electron tomographs of Coxsackievirus A9 treated with endosomal ionic condition buffer

Entry authors:
Domanska A , Plavec Z , Ruokolainen V, Löflund B, Marjomäki V, Butcher SJ 
Publication:
Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release
Domanska A , Plavec Z , Ruokolainen V, Löflund B, Marjomäki V, Butcher SJ 
J Virol 96 (2022)
PMID: 36448797
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-15706 (3.3Å)
Deposited:
2022-12-12
Released:
2023-01-20
Last modified:
2023-01-20
Dataset size:
1.8 TB
Dataset DOI:
10.6019/EMPIAR-11364
Experimental metadata:
Download xml
Contains:
micrographs
Image not available

Image set

1. Raw micrographs of CVA9 treated with ionic buffer condtions 3h
Category:micrographs - multiframe
Image format:MRC
No. of images or tilt series:1964
Frames per image:30
Image size:(4096, 4096)
Pixel type:SIGNED 16 BIT INTEGER
Pixel spacing:(1.24 Å, 1.24 Å)
Details:
.Micrographs were collected on FEI TAlos Arctica operating at 200 kV with a Falcon III direct electron detector at a nominal magnification of ×120,000. Each movie consisted of 30 frames with an exposure time of 1 s per frame. The total electron dose was approximately 30 electrons per Å2.
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