EMPIAR-11398

Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology

Entry authors:
Khavnekar S , Vrbovská V, Zaoralová M, Kelley R, Beck F, Kotecha A, Plitzko JM, Erdmann PS
Publication:
Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology
Khavnekar S , Vrbovská V, Zaoralová M, Kelley R, Beck F, Klumpe S , Kotecha A, Plitzko J , Erdmann PS 
bioRxiv (2022)
Experiment type:
EMDB
Related EMDB entry:
.EMD-16537 (4.6Å)
Deposited:
2023-01-22
Released:
2023-12-12
Last modified:
2023-12-12
Dataset size:
1.5 TB
Dataset DOI:
10.6019/EMPIAR-11398
Experimental metadata:
Download xml
Contains:
tilt series
Image not available

Image sets

1. Cryo-FIB milled S. Cerevisiae with post Platinum sputter coating
Category:tilt series
Image format:MRC
No. of images or tilt series:171
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.6 Å, 1.6 Å)
Details:
.Minimal Tomoman-Project
Use TOMOMAN preprocessing package for further processing, generation of tomograms etc.
TOMOMAN: DOI 10.5281/zenodo.4110736
https://github.com/sagarbiophysics/TOMOMAN/releases/tag/v0.6.14
2. Cryo-FIB milled S. Cerevisiae without post Platinum sputter coating
Category:tilt series
Image format:MRC
No. of images or tilt series:92
Image size:(4096, 4096)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.6 Å, 1.6 Å)
Details:
.Minimal Tomoman-Project
Use TOMOMAN preprocessing package for further processing, generation of tomograms etc.
TOMOMAN: DOI 10.5281/zenodo.4110736
https://github.com/sagarbiophysics/TOMOMAN/releases/tag/v0.6.14
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