EMPIAR-11751

Micrographs of ER-derived vesicles from HEK293F cells

Entry authors:
Gemmer M , Chaillet ML , van Loenhout J, Cuevas Arenas R , Vismpas D, Gröllers-Mulderij M, Koh FA, Albanese P, Scheltema RA , Howes SC, Kotecha A , Fedry J , Förster F 
Publication:
Visualization of translation and protein biogenesis at the ER membrane
Gemmer M , Chaillet ML , van Loenhout J, Cuevas Arenas R , Vismpas D, Gröllers-Mulderij M, Koh FA, Albanese P, Scheltema RA , Howes SC, Kotecha A , Fedry J , Förster F 
Nature 614 160-167 (2023)
PMID: 36697828
Experiment type:
EMDB
Related PDB entry:
Related EMDB entries:
.EMD-15870 (7.6Å), EMD-15872 (4.6Å), EMD-15871 (4.8Å), EMD-15884 (4.5Å), EMD-15879 (6.5Å), EMD-15875 (6.1Å), EMD-15880 (6.7Å), EMD-15874 (6.7Å), EMD-15877 (6.8Å), EMD-15876 (4.8Å)
Deposited:
2023-09-20
Released:
2023-11-06
Last modified:
2023-11-06
Dataset size:
1.1 TB
Funding:
.
1. European Research Council (ERC)
724425
Netherlands
2. Netherlands Organisation for Scientific Research (NWO)
724.016.001
Netherlands
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Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Unaligned gain-corrected micrographs of tilt-series on ER-derived vesicles from HEK293F cells
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:893
Frames per image:8
Image size:(3838, 3710)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(1.724 Å, 1.724 Å)
Details:
.Micrographs of ER-derived vesicles from HEK293F cells. Tilt-series collected between +-51 degrees with a 3 degree increment (35 tilts) and cumulative dose of less than 80 e-/A^2. Each tilt was collected as a movie with 8 frames and pixel size of 1.724 A/px.
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