EMPIAR-11797

Single-Particle Cryo-EM of RNA Polymerase at Various Tilts

Publication:
Overcoming Resolution Attenuation During Tilted Cryo-EM Data Collection
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
Deposited:
2023-11-27
Released:
2024-03-26
Last modified:
2024-03-26
Dataset size:
487.4 GB
Funding:
.
1. National Institutes of Health (NIH)
U54 AI170855
United States
2. National Institutes of Health (NIH)
U01 AI136680
United States
3. National Institutes of Health (NIH)
GM148476
United States
4. National Institutes of Health (NIH)
GM082946
United States
5. National Science Foundation (NSF, United States)
MCB 2048095
United States
6. Other private
Hearst Foundations Developmental Chair
United States
7. Other private
Margaret T. Morris Foundation
United States
8. National Research Foundation (NRF, Singapore)
NRF Fellowship A-8001346-00-00
Singapore
9. Other government
National University of Singapore (PYP Fellowship A-0008405-00-00, A-0008405-01-00)
Singapore
10. Ministry of Education (MoE, Singapore)
MOE AcRF Tier 1 A-8000037-00-00
Singapore
11. Ministry of Education (MoE, Singapore)
MOE AcRF Tier 2 A-6100427-01-00
Singapore
12. Other government
Agency for Science, Technology and Research, Singapore
Singapore
13. Damon Runyon Cancer Research Foundation
Nadia’s Gift Foundation Innovator Award DRR-65-21
United States
14. American Cancer Society
PF-21-075-01-CCB
United States
15. Other government
Cancer Center Support Grant P30 CA01495t
United States
16. National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM103310
United States
17. Simons Foundation
SF349247
United States
18. Other government
NYSTAR
United States
19. Other government
S10 OD032467
United States
20. National Science Foundation (NSF, United States)
MCB-1939086
United States
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Experimental metadata:
Contains:
micrographs
picked particles
Image not available

Image sets

1. Unaligned multiframe movies of RNA polymerase collected at various tilts
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:1146
Frames per image:51
Image size:(4092, 5760)
Pixel type:UNSIGNED BYTE
Pixel spacing:(0.83 Å, 0.83 Å)
Details:
.Movies collected using Titan Krios and K3 quantum with energy filter. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders. 50 frames were used, pixel size is 0.830 Å/pixel, total dose of 34.2 e-/Å2 (0 degrees) and 38.8 e-/Å2 (60 degrees). Gain reference is 22nov04c_04153642_01_4092x5760_norm_0.mrc.
2. Aligned and dose-weighted micrographs of RNA polymerase collected at various tilts
Category:micrographs - single frame
Image format:MRC
No. of images or tilt series:1146
Image size:(4092, 5760)
Pixel type:32 BIT FLOAT
Pixel spacing:(0.83 Å, 0.83 Å)
Details:
.Aligned and dose weighted using UCSF MotionCor2. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders.
3. Polished and equalized particles of RNA polymerase collected at various tilts
Category:picked particles - multiframe - processed
Image format:MRCS
No. of images or tilt series:2
Frames per image:72000
Image size:(312, 312)
Pixel type:32 BIT FLOAT
Pixel spacing:(0.83 Å, 0.83 Å)
Details:
.Final particle stack after particle polishing using Relion. The particles are equalized so that there are same number of particles per tilt angle with similar defocus distribution.
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