EMPIAR-11801

AL amyloid fibril from the FOR103 light chain

Publication:
Light chain mutations contribute to defining the fibril morphology in systemic AL amyloidosis
Karimi-Farsijani S , Pfeiffer PB , Banerjee S, Baur J, Kuhn L, Kupfer N, Hegenbart U, Schönland SO, Wiese S, Haupt C, Schmidt M , Fändrich M 
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-19818 (2.92Å)
Deposited:
2023-11-27
Released:
2024-09-03
Last modified:
2024-09-03
Dataset size:
504.3 GB
Funding:
.
1. German Research Foundation (DFG)
FA 456/23
Germany
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Unaligned multi-frame micrographs of FOR103 amyloid fibrils
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:2788
Frames per image:40
Image size:(3838, 3710)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(1.04 Å, 1.04 Å)
Details:
.Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 650nm
c2=50 objective_aperture=100

GIF-K2 counting
10s exposure 40 frames
total dose at the sample 53.70e/A2
1 pixel = 1.04A
dose rate ~3-4 e/pix/s

defocus range -1.2 to -2.5

The gain reference (/data/micrographs/CountRef_map_0076_001.dm4) is not applied to the micrographs. The NOTES.txt file contains EM information. Thumbnails and power spectra (data/thumbs) as well as coordinates (/data/Manual_Pick) are provided.
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