EMPIAR-11813

Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6

Entry authors:
Publication:
Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses
Proc Natl Acad Sci U S A 121 (2024)
PMID: 38427602
Experiment type:
EMDB
Related EMDB entry:
.EMD-42821 (7.8Å)
Deposited:
2023-11-28
Released:
2024-04-02
Last modified:
2024-04-02
Dataset size:
2.8 TB
Funding:
.
1. National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35GM122579
United States
2. Howard Hughes Medical Institute (HHMI)
N/A
United States
3. National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24GM129541
United States
4. National Science Foundation (NSF, United States)
GRFPDGE1656518
United States
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Experimental metadata:
Contains:
micrographs
picked particles
Image not available

Image sets

1. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 RNA.
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:1763
Frames per image:40
Image size:(4096, 4096)
Pixel type:SIGNED 16 BIT INTEGER
Pixel spacing:(1 Å, 1 Å)
Details:
.These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 8.50 seconds. Desired defocuses ranged from -1.2 to -2.5 nm. The exposures are gain corrected.
2. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 RNA.
Category:picked particles - single frame - processed
Image format:MRC
No. of images or tilt series:99785
Image size:(420, 420)
Pixel type:32 BIT FLOAT
Pixel spacing:(1 Å, 1 Å)
Details:
.Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/SARS-CoV-2_SL5-6/particles/P3_J1399_particles_exported.cs.
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