EMPIAR-12319

CryoEM of E. coli RNA polymerase on T7A1 promoter DNA

Entry authors:
Saecker RM , Chen J , Chiu CE, Malone B , Sotiris J, Ebrahim M, Yen LY , Eng ET , Darst SA 
Publication:
Structural origins of Escherichia coli RNA polymerase open promoter complex stability
Saecker RM , Chen J , Chiu CE, Malone B , Sotiris J, Ebrahim M, Yen LY , Eng ET , Darst SA 
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34599106
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-23897 (2.9Å)
Deposited:
2024-10-03
Released:
2024-12-21
Last modified:
2024-12-21
Dataset size:
1.6 TB
Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Unaligned raw movies
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:6790
Frames per image:30
Image size:(7420, 7676)
Pixel type:UNSIGNED BYTE
Pixel spacing:(1.06 Å, 1.06 Å)
Details:
.FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 7.24 e-/ A^2/s; pixel size 1.06 A/px(detector); pixel size 0.53 A/px(image); exposure 6 s; frame time 0.2 s; total dose 43.45 e-/A^2; total frames 30; defocus range -1.1 to -2.4 um; gain reference file 18nov08d_08110340_01_7676x7420_norm_0_mod.mrc is in references folder
XAvailable download options:
Loading...

Browse All Files

empair
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.