EMPIAR-12323

Unaligned multi-frame micrographs of the mu opioid receptor bound to a nanobody antagonist NbE

Entry authors:
Yu J , Kumar A , Zhang X , Martin C , Holsbeeck KV, Raia P , Koehl A, Laeremans T, Steyaert J, Manglik A , Ballet S , Boland A , Stoeber M 
Publication:
Structural basis of μ-opioid receptor targeting by a nanobody antagonist
Yu J, Kumar A, Zhang X , Martin C, Van Holsbeeck K , Raia P , Koehl A, Laeremans T, Steyaert J , Manglik A , Ballet S , Boland A , Stoeber M 
Nat Commun 15 (2024)
PMID: 39384768
Experiment type:
EMDB
Related PDB entry:
Related EMDB entry:
.EMD-18541 (3.2Å)
Deposited:
2024-09-04
Released:
2025-01-09
Last modified:
2025-01-09
Dataset size:
8.0 TB
Funding:
.
1. Swiss National Science Foundation
TMSGI3_211581
Switzerland
2. Swiss National Science Foundation
310030185235
Switzerland
3. Swiss National Science Foundation
PCEFP3_181282
Switzerland
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Experimental metadata:
Contains:
micrographs
Image not available

Image set

1. Unaligned multi-frame micrographs of the µ-opioid receptor bound to a nanobody antagonist NbE
Category:micrographs - multiframe
Image format:MRC
No. of images or tilt series:5939
Frames per image:44
Image size:(4096, 4096)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(0.9759 Å, 0.9759 Å)
Details:
.1. All the images are raw data.

2. All the images are multi-frame micrographs composed of 44 frames each in MRC format.

3. All the images are acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV using a Falcon III direct electron detector at a nominal
magnification of 150,000x, resulting in a pixel size of 0.9759 Å.

4. Data were collected using EPU with a defocus range of −0.6 to −2.0 μm and a total electron dose of 40 e−/Å2
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