EMPIAR-12463

Cryo-ET tilt series of FIB-milled Nup133-deficient mouse neural progenitor cells

Publication:
Nuclear pores safeguard the integrity of the nuclear envelop
Experiment type:
EMDB
Related EMDB entries:
.EMD-52164 (33.8Å), EMD-52163 (30.2Å), EMD-52162 (34.2Å)
Deposited:
2024-11-19
Released:
2025-01-29
Last modified:
2025-01-29
Dataset size:
360.0 GB
Experimental metadata:
Contains:
tilt series
Image not available

Image set

1. Cryo-ET tilt series of FIB-milled Nup133-deficient mouse neural progenitor cells
Category:tilt series
Image format:MRC
No. of images or tilt series:144
Image size:(5760, 4092)
Pixel type:SIGNED 16 BIT INTEGER
Pixel spacing:(2.682 Å, 2.682 Å)
Details:
.Images were recorded as movies of 10 frames and were motion-corrected on-the-fly in SerialEM. Tilt series were acquired from the stage pretilt angle of 8 degree, and images were collected using a dose-symmetric acquisition scheme with 2 degree increments, up to +64 or 66 and -52 degrees.
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