EMPIAR-10640

Cryo-EM of Multiple System Atrophy seeded assembly of alpha-synuclein filaments

Entry authors:
Publication:
Seeded assembly in vitro does not replicate the structures of α-synuclein filaments from multiple system atrophy
FEBS Open Bio (2021)
PMID: 33548114
Experiment type:
EMDB
Related PDB entries:
Related EMDB entries:
.EMD-12265 (3.43Å), EMD-12267 (3.55Å), EMD-12269 (3.18Å), EMD-12266 (3.84Å), EMD-12264 (3.47Å), EMD-12268 (4.23Å)
Deposited:
2021-02-11
Released:
2021-03-05
Last modified:
2021-03-05
Dataset size:
1.1 TB
Dataset DOI:
10.6019/EMPIAR-10640
Experimental metadata:
Download xml
Contains:
micrographs
picked particles
Image not available

Image sets

1. Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:2175
Frames per image:40
Image size:(3838, 3710)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(1.1 Å, 1.1 Å)
Details:
.Unaligned and non-gain corrected, 40-frame movies (K2 counting mode) were collected with EPU using a Titan Krios operating at 300 kV (LMB Krios II). The exposure was at 36.7 e/A^2) with a defocus range of -2.8 to -1.5 um. Micrographs were manually picked in relion using start-end helical picking. Gain reference is in Movies/Case1-gain-ref.MRC. Have fun.
2. Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:1294
Frames per image:40
Image size:(3838, 3710)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(1.14 Å, 1.14 Å)
Details:
.Unaligned and non-gain corrected, 40-frame movies (K2 counting mode) were collected with EPU using a Titan Krios operating at 300 kV (LMB Krios I). The exposure was at 32.6 e/A^2) with a defocus range of -2.8 to -1.5 um. Micrographs were manually picked in relion using start-end helical picking. Gain reference is in Movies/Case2-gain-ref.MRC. Have fun twice.
3. Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 5
Category:micrographs - multiframe
Image format:TIFF
No. of images or tilt series:1265
Frames per image:40
Image size:(3838, 3710)
Pixel type:UNSIGNED 16 BIT INTEGER
Pixel spacing:(1.14 Å, 1.14 Å)
Details:
.Unaligned and non-gain corrected, 40-frame movies (K2 counting mode) were collected with EPU using a Titan Krios operating at 300 kV (LMB Krios I). The exposure was at 37.5 e/A^2) with a defocus range of -2.8 to -1.5 um. Micrographs were manually picked in relion using start-end helical picking. Gain reference is in Movies/Case5-gain-ref.MRC. Have fun thrice.
4. Type 1A particles after Bayesian polishing
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:67619
Image size:(256, 256)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.14 Å, 1.14 Å)
Details:
.Polished particles for Type1A
5. Type 2A particles after Bayesian polishing
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:52502
Image size:(256, 256)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.14 Å, 1.14 Å)
Details:
.Polished particles for Type 2A
6. Type 1B particles after Bayesian polishing
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:33479
Image size:(256, 256)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.1 Å, 1.1 Å)
Details:
.Polished particles for Type 1B
7. Type 2B particles after Bayesian polishing
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:87092
Image size:(256, 256)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.1 Å, 1.1 Å)
Details:
.Polished particles for Type 2B
8. Type 2AB particles after Bayesian polishing
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:57358
Image size:(256, 256)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.1 Å, 1.1 Å)
Details:
.Polished particles for Type 2AB
9. Type 3 particles after Bayesian polishing
Category:picked particles - single frame - processed
Image format:MRCS
No. of images or tilt series:71131
Image size:(256, 256)
Pixel type:32 BIT FLOAT
Pixel spacing:(1.14 Å, 1.14 Å)
Details:
.Polished particles for Type 3
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