EMPIAR-10796 A microtubule RELION-based pipeline for cryo-EM image processing

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EMPIAR-10796

A microtubule RELION-based pipeline for cryo-EM image processing

Entry authors:

Cook AD , Manka SW , Wang S , Moores CA , Atherton J

Publication:

A microtubule RELION-based pipeline for cryo-EM image processing

Cook AD , Manka SW , Wang S , Moores CA , Atherton J

JBC (2019)

DOI: 10.1016/j.jsb.2019.10.004

Experiment type:

EMDB

Related EMDB entry:

EMD-10131 (4.2Å)

Deposited:

2019-07-24

Released:

2021-09-24

Last modified:

2022-02-04

Dataset size:

256.7 GB

Dataset DOI:

10.6019/EMPIAR-10796

Experimental metadata:

Contains:

micrographs

Image not available

Image sets

1. Movies of microtubules decorated with MKLP2 motor domain (ADPAlFx bound)

Category:micrographs - single frame

Image format:TIFF

No. of images or tilt series:293

Image size:(4880, 3600)

Pixel type:UNSIGNED 16 BIT INTEGER

Pixel spacing:(1.53 Å, 1.53 Å)

Details:

22 frames over 1.5 seconds (frames tiff format, dark and gain reference frames provided for correction).
50 electrons per squared Angstrom total dose over the whole movie.
Pixel size 1.53 Angstrom/Pixel.
Camera and microscope: DE20 direct detector in linear mode on a Technai Polara at 300kV.

data 256.7 GB

2. Single-frames of microtubules decorated with MKLP2 motor domain (ADPAlFx bound)

Category:micrographs - single frame

Image format:TIFF

No. of images or tilt series:6741

Image size:(5120, 3840)

Pixel type:UNSIGNED 16 BIT INTEGER

Pixel spacing:(1.53 Å, 1.53 Å)

Details:

22 frames over 1.5 seconds (frames tiff format, dark and gain reference frames provided for correction).
50 electrons per squared Angstrom total dose over the whole movie.
Pixel size 1.53 Angstrom/Pixel.
Camera and microscope: DE20 direct detector in linear mode on a Technai Polara at 300kV.

data 256.7 GB

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10796 256.7 GB

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