EMPIAR-10923 Cryo-electron tomographs of mouse hippocampal neurons

Cryo-electron tomographs of mouse hippocampal neurons

Entry authors:

Nedozralova H , Basnet N , Ibiricu I, Bodakuntla S , Biertümpfel C , Mizuno N

Publication:

In situ cryo-electron tomography reveals local cellular machineries for axon branch development

Nedozralova H , Basnet N , Ibiricu I, Bodakuntla S , Biertümpfel C , Mizuno N

J Cell Biol 221 (2022)

PMID: 35262630

DOI: 10.1083/jcb.202106086

Experiment type:

EMDB

Related EMDB entries:

EMD-25485, EMD-25486

Deposited:

2021-12-23

Released:

2022-03-04

Last modified:

2022-10-10

Dataset size:

88.6 GB

Dataset DOI:

10.6019/EMPIAR-10923

Experimental metadata:

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Contains:

reconstructed volumes

Image set

1. Reconstructed 4x binned tomograms of hippocampal neurons.

Category:reconstructed volumes

Image format:MRC

No. of images or tilt series:63

Frames per image:10

Image size:(928, 928)

Pixel type:32 BIT FLOAT

Pixel spacing:(21.8 Å, 21.8 Å)

Details:

Tilt-series were filtered according to the cumulative radiation dose (Grant and Grigorieff, 2015) and aligned on the basis of the patch tracking algorithm using the IMOD ETOMO package (Kremer et al., 1996). Tomograms were reconstructed from aligned stacks using weighted back-projection in IMOD. Tomograms were further 4x binned, resulting in pixel sizes of 21.8 Å. Tomograms were denoised by edge enhancing diffusion (bnad command in Bsoft (Heymann et al., 2008)).

TS_01-65_hip_bin4 88.6 GB

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10923 88.6 GB

EMPIAR-10923

Cryo-electron tomographs of mouse hippocampal neurons

Image set

1. Reconstructed 4x binned tomograms of hippocampal neurons.

Browse All Files

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