EMPIAR-11264

Cryo-electron tomography of microtubules assembled from purified porcine brain tubulin in the presence of GMPCPP

Entry authors:
Publication:
Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in Xenopus egg cytoplasmic extracts
Guyomar C , Bousquet C , Ku S, Heumann J, Guilloux G , Gaillard N , Heichette C, Duchesne L , Steinmetz MO , Gibeaux R , Chrétien D 
bioRxiv (2021)
Experiment type:
EMDB
Related EMDB entry:
.EMD-15740 (43.5Å)
Related EMPIAR entry:
EMPIAR-11253
Deposited:
2022-08-29
Released:
2022-11-14
Last modified:
2023-01-12
Dataset size:
76.1 GB
Dataset DOI:
10.6019/EMPIAR-11264
Experimental metadata:
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Contains:
tilt series
Image not available

Image set

1. Single- and dual-axis tilt series used to perform segmented sub-tomogram averaging of microtubules assembled in the presence of GMPCPP and decorated with kinesin motor domains
Category:tilt series
Image format:MRC
No. of images or tilt series:8
Pixel type:SIGNED 16 BIT INTEGER
Details:
.Single- and dual-axis tilt series. The number of images in the tilt series is variable.
GMPCPP_S1_tomo1, GMPCPP_S1_tomo2, GMPCPP_S1_tomo3 and GMPCPP_S1_tomo4 are dual-axis tilt series, with a pixel spacing of 7.9 Å (image size 2048, 2048).
GMPCPP_S2_tomo1 and GMPCPP_S2_tomo2 are single-axis tilt series, with a pixel size of 3.7 Å (image size 4096,4096).
GMPCPP_tomoFig5 and GMPCPP_tomoFig6 are single-axis tilt series, with a pixel size of 2.1 Å (image size 4096,4096).
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