EMPIAR-11786 SpCas9 bound to 10 nucleotide complementary DNA substrate

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EMPIAR-11786

SpCas9 bound to 10 nucleotide complementary DNA substrate

Entry authors:

Pacesa M , Loeff L , Querques I , Muckenfuss LM , Sawicka M , Jinek M

Publication:

R-loop formation and conformational activation mechanisms of Cas9

Pacesa M , Loeff L , Querques I , Muckenfuss LM , Sawicka M , Jinek M

Nature 609 191-196 (2022)

DOI: 10.1038/s41586-022-05114-0

Experiment type:

EMDB

Related PDB entry:

Related EMDB entry:

EMD-14500 (3.81Å)

Deposited:

2023-11-10

Released:

2023-12-11

Last modified:

2023-12-11

Dataset size:

157.5 GB

Dataset DOI:

10.6019/EMPIAR-11786

Funding:

1. Swiss National Science Foundation

31003A_182567

Switzerland

Experimental metadata:

Contains:

micrographs

Image not available

Image sets

1. Unaligned multi-frame micrographs of SpCas9 bound to 10 nucleotide complementary DNA substrate

Category:micrographs - multiframe

Image format:TIFF

No. of images or tilt series:597

Frames per image:36

Image size:(11520, 8184)

Pixel type:UNSIGNED BYTE

Pixel spacing:(0.325 Å, 0.325 Å)

Details:

Unaligned multi-frame movies with each containing 36 frames per movie collected at 300 kV in super-resolution counting mode. Total accumulated electron exposure on the specimen level corresponds to 66.6 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU.

20211005 157.5 GB

2. Gain reference

Category:micrographs - single frame

Image format:DM4

No. of images or tilt series:1

Image size:(11520, 8184)

Pixel type:32 BIT FLOAT

Pixel spacing:(0.325 Å, 0.325 Å)

Details:

Gain reference

20211004_gain_ref 699.3 MB

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11786 157.5 GB

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