ID   DAA08429; SV 1; linear; genomic DNA; STD; FUN; 894 BP.
XX
PA   BK006942.2
XX
PR   Project:PRJNA43747;
XX
DT   08-JUN-2010 (Rel. 105, Created)
DT   29-JUN-2024 (Rel. 144, Last updated, Version 35)
XX
DE   Saccharomyces cerevisiae S288C acylglycerone-phosphate reductase
XX
KW   TPA; TPA:inferential.
XX
OS   Saccharomyces cerevisiae S288C
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
XX
RN   [1]
RX   PUBMED; 8849441.
RA   Goffeau A., Barrell B.G., Bussey H., Davis R.W., Dujon B., Feldmann H.,
RA   Galibert F., Hoheisel J.D., Jacq C., Johnston M., Louis E.J., Mewes H.W.,
RA   Murakami Y., Philippsen P., Tettelin H., Oliver S.G.;
RT   "Life with 6000 genes";
RL   Science 274(5287):.
XX
RN   [2]
RX   PUBMED; 9169870.
RA   Churcher C., Bowman S., Badcock K., Bankier A., Brown D., Chillingworth T.,
RA   Connor R., Devlin K., Gentles S., Hamlin N., Harris D., Horsnell T.,
RA   Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C., Pearson D.,
RA   Rajandream M., Rice P., Rowley N., Skelton J., Smith V., Barrell B. et al.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX";
RL   Nature 387(6632 SUPPL):84-87(1997).
XX
RN   [3]
RX   PUBMED; 34897464.
RA   Engel S.R., Wong E.D., Nash R.S., Aleksander S., Alexander M., Douglass E.,
RA   Karra K., Miyasato S.R., Simison M., Skrzypek M.S., Weng S., Cherry J.M.;
RT   "New data and collaborations at the Saccharomyces Genome Database: updated
RT   reference genome, alleles, and the Alliance of Genome Resources";
RL   Genetics 220(4):(2022).
XX
RN   [4]
RG   Saccharomyces Genome Database
RA   ;
RT   ;
RL   Submitted (14-DEC-2009) to the INSDC.
RL   Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
XX
RN   [5]
RC   Sequence update by submitter
RG   Saccharomyces Genome Database
RA   ;
RT   ;
RL   Submitted (31-MAR-2011) to the INSDC.
RL   Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
XX
RN   [6]
RC   Protein update by submitter
RG   Saccharomyces Genome Database
RA   ;
RT   ;
RL   Submitted (04-MAY-2012) to the INSDC.
RL   Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
XX
DR   MD5; 8a39b675dc3b3db9b9f565b8ed04fc42.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..894
FT                   /organism="Saccharomyces cerevisiae S288C"
FT                   /chromosome="IX"
FT                   /strain="S288C"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:559292"
FT   CDS             BK006942.2:126204..127097
FT                   /codon_start=1
FT                   /gene="AYR1"
FT                   /gene_synonym="GBG1"
FT                   /locus_tag="YIL124W"
FT                   /product="acylglycerone-phosphate reductase"
FT                   /EC_number="1.1.1.101"
FT                   /EC_number="3.1.1.3"
FT                   /note="Bifunctional triacylglycerol lipase and 1-acyl DHAP
FT                   reductase; NADPH-dependent 1-acyl dihydroxyacetone
FT                   phosphate reductase involved in phosphatidic acid
FT                   biosynthesis; lipid droplet triacylglycerol lipase involved
FT                   in mobilization of non-polar lipids; found in lipid
FT                   particles, endoplasmic reticulum and mitochondrial outer
FT                   membrane; forms NADPH-regulated channel in mitochondrial
FT                   outer membrane; required for spore germination; role in
FT                   cell wall biosynthesis; capable of metabolizing steroid
FT                   hormones"
FT                   /experiment="EXISTENCE:direct assay:GO:0004806 triglyceride
FT                   lipase activity [PMID:24187129]"
FT                   /experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
FT                   [PMID:11914276]"
FT                   /experiment="EXISTENCE:direct assay:GO:0005741
FT                   mitochondrial outer membrane [PMID:16407407]"
FT                   /experiment="EXISTENCE:direct assay:GO:0005811 lipid
FT                   droplet [PMID:10617610|PMID:24868093]"
FT                   /experiment="EXISTENCE:genetic interaction:GO:0004806
FT                   triglyceride lipase activity [PMID:24187129]"
FT                   /experiment="EXISTENCE:genetic interaction:GO:0019433
FT                   triglyceride catabolic process [PMID:24187129]"
FT                   /experiment="EXISTENCE:mutant phenotype:GO:0000140
FT                   acylglycerone-phosphate reductase activity [PMID:10617610]"
FT                   /experiment="EXISTENCE:mutant phenotype:GO:0004806
FT                   triglyceride lipase activity [PMID:24187129]"
FT                   /experiment="EXISTENCE:mutant phenotype:GO:0006654
FT                   phosphatidic acid biosynthetic process [PMID:10617610]"
FT                   /experiment="EXISTENCE:direct assay:GO:0005739
FT                   mitochondrion [PMID:16823961|PMID:24769239|PMID:14576278]"
FT                   /experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
FT                   reticulum [PMID:26928762|PMID:10617610]"
FT                   /protein_id="DAA08429.1"
FT                   /translation="MSELQSQPKKIAVVTGASGGIGYEVTKELARNGYLVYACARRLEP
FT                   MAQLAIQFGNDSIKPYKLDISKPEEIVTFSGFLRANLPDGKLDLLYNNAGQSCTFPALD
FT                   ATDAAVEQCFKVNVFGHINMCRELSEFLIKAKGTIVFTGSLAGVVSFPFGSIYSASKAA
FT                   IHQYARGLHLEMKPFNVRVINAITGGVATDIADKRPLPETSIYNFPEGREAFNSRKTMA
FT                   KDNKPMPADAYAKQLVKDILSTSDPVDVYRGTFANIMRFVMIFVPYWLLEKGLSKKFKL
FT                   DKVNNALKSKQKNKDD"
XX
SQ   Sequence 894 BP; 271 A; 165 C; 199 G; 259 T; 0 other;
     atgtcggagt tacagtcaca acctaaaaag attgccgttg ttacaggcgc ctcaggtggt        60
     attggatatg aggtaacgaa ggaattagcc agaaatggct atttggttta tgcatgtgca       120
     agaagactag aaccaatggc acaattggcc atccaatttg gtaacgatag tatcaaacca       180
     tataagttgg atatttccaa gccagaagaa attgtaacat tttctggttt tttgcgagct       240
     aatttacctg atggtaaatt agatttattg tacaacaatg ccggtcagag ttgtacattc       300
     cctgcattgg atgcaacaga tgctgcagta gaacaatgct ttaaggtcaa tgtgtttggt       360
     catatcaaca tgtgccgtga attgtcggag ttccttatta aagcgaaagg aaccattgtc       420
     ttcactggtt ccttggcagg agttgtgtca ttcccctttg gtagcattta ttccgcttct       480
     aaagctgcta tccaccaata tgctcgtggg ttacatctag agatgaagcc cttcaacgtg       540
     agagtgatta acgctattac tggcggtgtg gctactgata tcgctgataa aaggcccttg       600
     cctgaaacct caatatacaa cttccctgaa ggtagggaag cttttaattc aagaaaaacc       660
     atggctaagg acaataagcc catgccagct gatgcgtacg caaagcaact ggttaaagat       720
     atcttgagca ctagtgaccc tgtagatgtt tatagaggaa cgttcgccaa catcatgcgt       780
     tttgtcatga tatttgttcc ttactggcta ttggaaaaag gactttccaa aaaatttaaa       840
     ttggacaaag ttaataatgc tttgaagtcg aaacagaaga ataaggacga ttag             894
//