ID   G26777; SV 1; linear; genomic DNA; STS; HUM; 561 BP.
XX
AC   G26777;
XX
DT   06-JUN-1996 (Rel. 47, Created)
DT   27-APR-2000 (Rel. 63, Last updated, Version 3)
XX
DE   human STS STS_M74826, sequence tagged site.
XX
KW   primer; sequence tagged site; STS; STS sequence.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-561
RA   Hudson T.;
RT   "Whitehead Institute/MIT Center for Genome Research; Physically Mapped
RT   STSs";
RL   Unpublished.
XX
DR   MD5; 1fbb80780c9a073dcb567d4c82e004bd.
XX
CC   Contact: Thomas Hudson
CC   Whitehead Institute/MIT Center for Genome Research
CC   Whitehead Institute for Biomedical Research
CC   9 Cambridge Center, Cambridge MA 02142 USA
CC   Tel: 617 252 1900
CC   Fax: 617 252 1902
CC   Email: thudson@genome.wi.mit.edu
CC   Primer A: ACAAAATGCAAGGTGAACACC
CC   Primer B: CACGTGCAAATGATACCCAG
CC   STS size: 200
CC   PCR Profile:
CC   Presoak:
CC   Denaturation:
CC   Annealing: 56 degrees C
CC   Polymerization:
CC   PCR Cycles: 35
CC   Thermal Cycler:
CC   Protocol:
CC   Template: 10 ng
CC   Primer: each 5 pM
CC   dNTPs: each 4 nM
CC   Taq Polymerase: 0.025 units/ul
CC   Total Vol: 20 ul
CC   Buffer:
CC   MgCl2: 1.5 mM
CC   KCl: 50 mM
CC   Tris-HCL: 10 mM
CC   pH: 9.3
CC   Derived from dbEST (genbank accession M74826).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..561
FT                   /organism="Homo sapiens"
FT                   /map="162.0 cR from top of Chr10 linkage group"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:9606"
FT   STS             98..297
FT   primer_bind     98..118
FT   primer_bind     complement(278..297)
XX
SQ   Sequence 561 BP; 179 A; 124 C; 100 G; 158 T; 0 other;
     taataacctt gctcaccaag ctgttccact tctctagaga acatgccctc agctaagccc        60
     cctactgaga aacttccttt gagaattgtg cgacttcaca aaatgcaagg tgaacaccac       120
     tttgtctctg agaacagacg ttaccaatta tggagtgtca ccagctgcca aaatcgtagg       180
     tgttggctct gctggtcact ggagtagttg ctactcttca gaatatggac aaagaaggca       240
     caggtgtaaa tatagtagca ggatgaggaa cctcaaactg ggtatcattt gcacgtgctc       300
     ttctgttctc aaatgctaaa tgcaaacact gtgtatttat tagttaggtg tgccaaacta       360
     ccgttcccaa attggtgttt ctgaatgaca tcaacattcc cccaacatta ctccattact       420
     aaagacagaa aaaaataaaa acataaaata tacaaacatg tggcaacctg ttcttcctac       480
     caaatataaa cttgtgtatg atccaagtat tttatctgtg ttgtctctct aaacccaaat       540
     aaatgtgtaa atgtggacac a                                                 561
//