ID   MBS0434012; SV 1; linear; genomic DNA; WGS; ENV; 1491 BP.
XX
PA   JAFEEE010000023.1
XX
PR   Project:PRJNA694732;
XX
DE   Proteobacteria bacterium (activated sludge metagenome)
DE   UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase
XX
KW   WGS.
XX
OS   Proteobacteria bacterium (activated sludge metagenome)
OC   Bacteria; Proteobacteria.
XX
RN   [1]
RX   PUBMED; 33819658.
RA   Wang Y., Zhao R., Liu L., Li B., Zhang T.;
RT   "Selective enrichment of comammox from activated sludge using antibiotics";
RL   Water Res 197:.
XX
RN   [2]
RA   Wang Y.;
RT   ;
RL   Submitted (29-JAN-2021) to the INSDC.
RL   Civil Engineering, The University Hong Kong, Pokfulam road, Hong Kong
RL   999077, Hong Kong
XX
DR   MD5; 54717bd40903ea6f5f319ea3ca2fd8cd.
DR   BioSample; SAMN17574120.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1491
FT                   /organism="Proteobacteria bacterium"
FT                   /environmental_sample
FT                   /isolate="SZAS AMP-54"
FT                   /mol_type="genomic DNA"
FT                   /country="China: Shenzhen"
FT                   /lat_lon="22.49 N 113.88 E"
FT                   /isolation_source="activated sludge"
FT                   /collection_date="2019-04"
FT                   /note="metagenomic"
FT                   /db_xref="taxon:1977087"
FT                   /submitter_seqid="PROT-AMP-7__23__length-44602"
FT                   /metagenome_source="activated sludge metagenome"
FT   CDS             complement(JAFEEE010000023.1:37370..38860)
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /locus_tag="JSR75_03820"
FT                   /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
FT                   6-diaminopimelate ligase"
FT                   /EC_number="6.3.2.13"
FT                   /note="Derived by automated computational analysis using
FT                   gene prediction method: Protein Homology."
FT                   /inference="COORDINATES: similar to AA
FT                   sequence:RefSeq:WP_015914266.1"
FT                   /protein_id="MBS0434012.1"
FT                   /translation="MAGLHTLQHLADTRAACEWLAHRGVRGLATDSRRVRPGDAFIAWP
FT                   GYALDGRQYVKAALAAGASACLVEADGVAGFDFGADAPVAALAGLKARTGEIADEFYGR
FT                   PSESLAVVATTGTNGKTSTAWWTAQALRQLGKRCGVIGTLGVGEPPQGLQFTGLTTPDP
FT                   VTVQQSLRRFADQGFAACAIEASSIGVVEQRLAGTRFAVALFTNFTQDHLDYHGSMAAY
FT                   WDAKRALFDWPGLRAAVINLDDPKGDELAAQLRGPEAWTYSLRRDARLRAERIAYADGG
FT                   LGFDVVEGAARAPVRSALIGEYNVSNLLAVIGGLRALGVPLAEAAAACAVLSSVPGRMQ
FT                   RVEASRAGPEVVVDYAHSPDALEKALIALRPFATQRGGRLWAVFGCGGNRDTAKRPLMG
FT                   AIAQRLADRVVLTSDNPRHEAPAAILAQIRAGLSGDAQAVKVIEDRRAAIVASVREAAP
FT                   ADVILLAGKGHEDYQEIAGVKQPFSDVAEARLALEALR"
XX
SQ   Sequence 1491 BP; 196 A; 549 C; 552 G; 194 T; 0 other;
     atggccggcc tgcacaccct tcaacatctg gccgacacgc gcgccgcctg cgaatggctg        60
     gcgcaccgcg gcgtgcgcgg cctggccacc gacagccgcc gcgtgcggcc gggcgacgcc       120
     ttcatcgcct ggccgggcta cgcgctcgac gggcgccagt acgtcaaggc ggcgctggcc       180
     gcaggcgcct cggcctgcct ggtcgaggcc gacggcgtgg ccggcttcga tttcggtgcc       240
     gatgcaccgg tggcggcgct cgcgggcctc aaggcccgca ccggcgagat cgccgacgag       300
     ttctacggcc ggccgagcga atcgctggcg gtggtggcca ccaccggcac caacggcaag       360
     acctcgacgg cctggtggac ggcgcaggcg ctgcgccagc tcggcaagcg ctgcggggtg       420
     atcggcacct tgggtgtcgg cgaaccaccg caaggcctgc agttcaccgg cctgaccacg       480
     cccgacccgg tgaccgtgca gcagtcgctg cgccgtttcg ccgatcaggg cttcgccgcc       540
     tgcgcgatcg aggcctcgtc gatcggcgtg gtcgagcagc gcctggccgg cacgcgcttc       600
     gcggtggcgc tgttcaccaa cttcacgcag gaccatctcg actaccacgg cagcatggcc       660
     gcctactggg acgccaagcg cgcgctgttc gactggccgg gcctgcgcgc cgcggtgatc       720
     aacctcgacg accccaaggg cgacgagctc gcggcgcagc tgcgcggccc cgaggcctgg       780
     acctactcgc tgcggcgcga tgcgcggctg cgcgccgagc gcatcgccta cgccgacggc       840
     gggctgggct tcgacgtggt cgagggcgcg gcgcgcgcgc cggtgcgcag cgcgctgatc       900
     ggcgaataca acgtctccaa cctgctggcc gtgatcggcg gcttgcgtgc gctcggcgta       960
     ccgctggccg aggccgccgc cgcctgcgcg gtgctgagct cggtgccggg gcgcatgcag      1020
     cgcgtcgaag cgtcgcgcgc cggccccgag gtggtggtcg actacgccca ttcgcccgac      1080
     gcgctcgaga aggcgctgat cgcgctgcgc cccttcgcca cgcagcgcgg cggccggctg      1140
     tgggcggtat tcggctgcgg cggcaaccgc gacaccgcca agcgtccgct gatgggcgcg      1200
     atcgcgcaac gcctggccga ccgcgtcgtg ctcaccagcg acaacccgcg ccacgaagcg      1260
     cctgcggcca tcctggcgca gatccgcgcc ggcctgagcg gcgatgcgca ggcggtgaag      1320
     gtgatcgagg atcggcgcgc ggccatcgtc gccagcgtgc gcgaggccgc gccggccgac      1380
     gtgatcctgc tggccggcaa gggccacgag gactaccagg agatcgcggg cgtgaagcag      1440
     ccgttctccg acgtggccga agcgcgcctc gcgctggagg cattgcgatg a               1491
//