ID   T67838; SV 1; linear; mRNA; EST; HUM; 390 BP.
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AC   T67838;
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DT   10-MAR-1995 (Rel. 43, Created)
DT   31-JAN-2011 (Rel. 107, Last updated, Version 5)
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DE   yc27f04.r1 Stratagene liver (#937224) Homo sapiens cDNA clone IMAGE:81919
DE   5', mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-390
RX   PUBMED; 8889549.
RA   Hillier L., Lennon G., Becker M., Bonaldo M.F., Chiapelli B., Chissoe S.,
RA   Dietrich N., DuBuque T., Favello A., Gish W., Hawkins M., Hultman M.,
RA   Kucaba T., Lacy M., Le M., Le N., Mardis E., Moore B., Morris M.,
RA   Parsons J., Prange C., Rifkin L., Rohlfing T., Schellenberg K.,
RA   Soares M.B., Tan F., Thierry-Meg J., Trevaskis E., Underwood K.,
RA   Wohldmann P., Waterston R., Wilson R., Marra M.;
RT   "Generation and analysis of 280,000 human expressed sequence tags";
RL   Genome Res. 6(9):807-828(1996).
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DR   MD5; d2fc28cfb10dafdcdf0042ded4b9b6bd.
DR   UNILIB; 501; 252.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   Insert Size: 834
CC   High qality sequence stops: 312 Source: IMAGE Consortium, LLNL This
CC   clone is available royalty-free through LLNL ; contact the IMAGE
CC   Consortium (info@image.llnl.gov) for further information.
CC   Insert Length: 834   Std Error: 0.00
CC   Seq primer: M13RP1
CC   High quality sequence stop: 312.
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FH   Key             Location/Qualifiers
FH
FT   source          1..390
FT                   /organism="Homo sapiens"
FT                   /lab_host="SOLR cells (kanamycin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="49 years old"
FT                   /clone_lib="LIBEST_000252 Stratagene liver (#937224)"
FT                   /clone="IMAGE:81919"
FT                   /note="Organ: liver; Vector: pBluescript SK; Site_1: EcoRI;
FT                   Site_2: XhoI; Cloned unidirectionally. Primer: Oligo dT.
FT                   Hepatectomy from normal male caucasian. Average insert
FT                   size: 1.1 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5'
FT                   GAATTCGGCACGAG 3' ~3' adaptor sequence: 5'
FT                   CTCGAGTTTTTTTTTTTTTTTTTT 3'"
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:501"
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SQ   Sequence 390 BP; 77 A; 103 C; 101 G; 102 T; 7 other;
     aaaacctcaa gagatagctt ngagggggtc agctgactct ggccatggac taaatgtaaa        60
     gtcggtgaat tttggcctct gtacatttag tgcactctct ggggaggtct gctatgtgtc       120
     tgacagtntg caagaaattg gggatatgtc cctaggattc ctccacttag cgccactggg       180
     ccctcccact cctgccatct caagtgacca tcgaccttac ccacggctgc tgcacctnac       240
     cctgctctgg tgcacattcc ccactgcagc atttgcacgt ggacagctgt tcccgggtgg       300
     ctgtttccag atcttcacca cagggttgcc tcttggtngt ttntggggca acagagtggg       360
     gacagggttt nttagacagg gcancacttt                                        390
//