ID   W18369; SV 1; linear; mRNA; EST; MUS; 377 BP.
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AC   W18369;
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DT   05-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 3)
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DE   mb97c08.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:337358 5'
DE   similar to gb:V00714 Mouse gene for alpha-globin (MOUSE);, mRNA sequence.
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KW   EST.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-377
RA   Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA   Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA   Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA   Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RT   "The WashU-HHMI Mouse EST Project";
RL   Unpublished.
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DR   MD5; f1ea5a1714ca377d833becb3e65cfaa3.
DR   UNILIB; 637; 431.
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CC   On May 9, 1995 this sequence version replaced gi:802379.
CC   Contact: Marra M/Mouse EST Project
CC   WashU-HHMI Mouse EST Project
CC   Washington University School of MedicineP
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: mouseest@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   MGI:218758
CC   Putative full length read
CC   Seq primer: mob.REGA+ET.
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FH   Key             Location/Qualifiers
FH
FT   source          1..377
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /dev_stage="19.5 dpc total fetus"
FT                   /clone_lib="Soares mouse p3NMF19.5"
FT                   /clone="IMAGE:337358"
FT                   /note="Vector: pT7T3D (Pharmacia) with a modified
FT                   polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
FT                   was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr.
FT                   Minoru Ko (Wayne State University)."
FT                   /db_xref="taxon:10090"
FT                   /db_xref="UNILIB:637"
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SQ   Sequence 377 BP; 77 A; 120 C; 102 G; 78 T; 0 other;
     gggggaagac aaaagcaaca tcaaggctgc ctgggggaag attggtggcc atggtgctga        60
     atatggagct gaagccctgg aaaggatgtt tgctagcttc cccaccacca agacctactt       120
     ccctcacttt gatgtaagcc acggctctgc ccaggtcaag ggtcacggca agaaggtcgc       180
     cgatgctctg gccaatgctg caggccacct cgatgacctg cccggtgccc tgtctgctct       240
     gagcgacctg catgcccaca agctgcgtgt ggatcccgtc aacttcaagc tcctgagcca       300
     ctgcctgctg gtgaccttgg ctagccacca ccctgccgat ttcacccccg cggtgcatgc       360
     ctctctggat aaattcc                                                      377
//