ID   W53392; SV 1; linear; mRNA; EST; MUS; 285 BP.
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AC   W53392;
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DT   06-JUN-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   md43d03.r1 Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA clone
DE   IMAGE:371141 5' similar to gb:M33374 CELL ADHESION PROTEIN (HUMAN);, mRNA
DE   sequence.
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KW   EST.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-285
RA   Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA   Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA   Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA   Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RT   "The WashU-HHMI Mouse EST Project";
RL   Unpublished.
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DR   MD5; 54b9dff4ad2a42cf8d86ee8f67b46458.
DR   UNILIB; 562; 437.
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CC   On Apr 14, 1993 this sequence version replaced gi:693525.
CC   Contact: Marra M/Mouse EST Project
CC   WashU-HHMI Mouse EST Project
CC   Washington University School of MedicineP
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: mouseest@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   MGI:232573
CC   Trace considered overall poor quality
CC   Seq primer: mob.REGA+ET
CC   High quality sequence stop: 1.
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FH   Key             Location/Qualifiers
FH
FT   source          1..285
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B"
FT                   /strain="C57BL/6J"
FT                   /mol_type="mRNA"
FT                   /sex="unknown"
FT                   /dev_stage="13.5-14.5dpc total fetus"
FT                   /clone_lib="Soares mouse embryo NbME13.5 14.5"
FT                   /clone="IMAGE:371141"
FT                   /tissue_type="embryo"
FT                   /note="Vector: pT7T3D-Pac (Pharmacia) with a modified
FT                   polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
FT                   was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTT
FT                   T 3'], on equal amounts of mRNA from 2 13.5dpc and 2
FT                   14.5dpc embryos [total RNA provided by  Minoru Ko, Wayne
FT                   State Univ., from 2 ]; double-stranded cDNA was ligated to
FT                   Eco RI adaptors (Pharmacia), digested with Not I and cloned
FT                   into the Not I and Eco RI sites of the modified pT7T3
FT                   vector. Library went through one round of normalization,
FT                   and was constructed by Bento Soares and M.Fatima Bonaldo. 
FT                   "
FT                   /db_xref="taxon:10090"
FT                   /db_xref="UNILIB:562"
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SQ   Sequence 285 BP; 66 A; 87 C; 78 G; 54 T; 0 other;
     ccacgggcgc gcaccttacc cctctctatc tgtgggatgc ctcggtggaa cctgaccccg        60
     agaagatacg cagcttcccg caggacctct ggtttccgta gcgcaaggag cgagtgatgg       120
     tggccataca acagtagatg atggctgccc agctgacact gcagcaacgt gattactgtc       180
     gcacactatc tcatcaggct gctgaagtgc gagcgagaca agttccgcaa cttcgtagcc       240
     tagcaagtac gagcagcacg actggcacta ctgcgagcac ctgca                       285
//