This study aimed to clarify the characteristics of cardiac neural crest cells during heart development at spatial level. The embryonic hearts labelled by Wnt1-Cre; R26-EYFP mice, which showed the population of the neural crest cells were used to analyze the spatial transcriptomics by using the technology of Visium at E14.5 and E17.5. This results reveals the spatiotemporal gene expression profiles originated from the approximately 5000 spots per each sections. Adjusting the gene expression matrixes to each HE staining data clarify the difference of cell characteristics depending on the histology. Furthermore, the analysis of spots transcriptome data reveal that the continuously of cell differentiation based on the histological validation.