The fission yeast Schizosaccharomyces pombe is an ideal model organism for studying epigenetics. As S. pombe typically grows as haploid cells and possesses a small genome (12.8 Mb), novel genetic variations corresponding to mutant phenotypes can be easily identified by whole genome sequencing. In this project, we first sequenced the genomes of a wild-type strain and a mutant strain possessing the rpb2-m203 mutation to confirm the ability of our pipeline to identify single nucleotide polymorphisms. Next, we sequenced a strain with no apparent phenotype that survived three rounds of UV irradiation to check the effect of UV irradiation. In a genetic screen, we isolated a silencing mutant named K20. The genome of the original isolate of K20 was sequenced to identify corresponding mutation.