Trichoplusia ni (High Five) insect derived cell-lines, is one of the most widely used systems for recombinant protein expression in Baculoviral Expression Vector System (BEVS). Genomic sequences is still absent for High Five. In this study we present an approach using different sequencing data types with short-read sequencing, long synthetic and Oxford Nanopore reads to build genomes at an unprecedented resolution. The High Five assembly contains 2,954 scaffolds of size, 332 Mb with N50 of 326 Kb.