We have investigated the human cytomegalovirus lytic transcriptome using the Pacific Biosciences Sequel (PacBio) and the Oxford Nanopore Technologies (ONT) MinION (R9.4) platform. The sample sequenced on the PacBio platform was prepared following the IsoSeq protocol. The samples sequenced on the MinION platform include a cDNA sample that was cap-selected and a cDNA sample that was not. One RNA sample was also sequenced by the MinION sequencer in its native RNA form using direct RNA sequencing.