Project: PRJEB34404
Aim: To investigate the effect of 7-day use of chlorhexidine (CHX) mouthwash on the salivary microbiome as well as several saliva and plasma biomarkers in healthy individuals.Material and Methods: Following a single blind, cross-over and non-randomized design we recruited 36 healthy individuals. They rinsed their mouth (for 1 min) twice a day for seven days with a placebo mouthwash and then repeated this protocol with CHX mouthwash for a further seven days. Saliva and blood samples were taken at the end of each treatment to analyse the abundance and diversity of oral bacteria, and pH, lactate, glucose, nitrate and nitrite concentrations.Results: CHX significantly increased the abundance of Firmicutes and Proteobacteria, and reduced the content of Bacteroidetes, TM7, SR1 and Fusobacteria. This shift was associated with a significant decrease in saliva pH and buffering capacity, accompanied by an increase in saliva lactate and glucose levels. Lower saliva and plasma nitrite concentrations were found after using CHX, followed by a trend of increased systolic blood pressure.Conclusions: Overall, this study demonstrates that mouthwash containing CHX causes a major shift in the salivary microbiome, leading to more acidic conditions and lower nitrite availability in healthy individuals.
Secondary Study Accession:
ERP117301
Study Title:
The effect of chlorhexidine mouthwash on the the oral microbiome
Center Name:
University of Plymouth
Study Name:
Chlorhexidine and oral microbiome
ENA-FIRST-PUBLIC:
2019-11-13
ENA-LAST-UPDATE:
2019-09-13
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