Project: PRJEB47633
The emergence of almond (Prunus amygdalus (L.) Batsch, syn P. dulcis (Mill.)) intensive and semi-intensive cropping systems has created a necessity for new almond cultivars with vigor and shape adapted to these new circumstances. Hence, it is important to unravel which mechanisms are behind the regulation of the tree three-dimensional structure, or tree architecture, and what factors may play a role, like the rootstock choice. In this study, we have analyzed the rootstock influence in the scion transcriptome, regarding the biological processes that control almond tree architecture. 3 commercial almond cultivars were grafted onto 3 hybrid rootstocks, resulting in 9 combinations, whose gene expression in shoot tips were analyzed via RNA-seq. We report that differences in tree architecture phenotype are correlated with differential expression of genes involved in hormonal and molecular responses associated with the regulation of apical dominance, branch formation, plant growth, cell wall formation or nitrogen assimilation. These results highlight the importance of the rootstock choice in the selection of a desirable scion architecture and in the establishment of almond orchards.
Secondary Study Accession:
ERP131922
Study Title:
Almond scion tree architecture is regulated by numerous processes. Rootstock genotype may influence the development of the scion vigor and shape. To gain insight into this regulation, we conducted a RNA-Seq analysis in shoot tips samples from 3 almond cultivars grafted onto 3 different rootstock.
Center Name:
CENTRO DE INVESTIGACION Y TECNOLOGIA AGROALIMENTARIA DE ARAGON
Study Name:
Identification of genes involved in almond scion tree architecture influenced by rootstock genotype using transcriptome analysis
End Date Of Study Co_715:0000254:
07/31/2021
Constact Institutions Co_715:0000075:
Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Unidad de Hortofruticultura, Gobierno de Aragón, Avda. Montañana 930, 50059 Zaragoza, Spain
Experimental Site Name Co_715:0000002:
Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA) experimental orchard El Vedado Bajo el Horno (Zuera, Zaragoza, 41°51'46.5"N 0°39'09.2"W)
Description Of The Experimental Design Co_715:0000003:
Three repilcates from different trees were sequenced for each combination
Cultural Practices Co_715:0000236:
Trees were planted as a single axe and supported by a wooden stake. Trees were then left without pruning so that, they could express their natural growth habit unaltered. Conventional orchard practices were used for weed control and drip irrigation. Soil type was calcareous with pH around 7-8.
ENA-FIRST-PUBLIC:
2022-03-20
General