To characterize changes in chromatin accessibility acorss the CRC malignancy continum, wild type organoids (WT), ApcKO (A) and ApcKO::KrasG12D::Smad4KO::Trp53KO (AKSP) tumoroids were subjected to bulk ATAC-seq. We then performed differential accessibility analysis using data obtained from ATAC-seq of the indicated organoid lines. Overall design: Wild type intestinal organoids (WT), ApcKO (A) and ApcKO::KrasG12D::Smad4KO::Trp53KO (AKSP) tumoroids were collected for nuclei isolation and DNA transpostion when they reached confluence.