We assembled a pseudo-chromosome genome of the allotetraploid grass Z. sinica using Nanopore and Illumina sequencing. The genome had 20 pseudomolecules with a total length of 313.7 Mb and high contiguity, as well as a scaffold N50 value of 18.9 Mb. We predicted 30,476 protein-coding genes based on full-length transcripts using PacBio long-read isoform sequencing and 41.64% of repeat sequences in the Z. sinica genome.