Comparison of expression in wild type ovules to that in dif1 mutant (no embro sac) and myb98 mutant (impaired pollen tube guidance) Keywords: gene expression analysis Overall design: 12 samples were analyzed, with replicates per genotpye (wild type, dif1, myb98). Ovules were dissected out of muture ovaries. Total RNA was isloated by spin column purification, 2 ug were used as template for reverse transcription, seceond strand synthesis and biotin labeling.