Domesticated herbivores are an important agricultural resource and play critical roles in global food security, particularly as they can adapt to varied environments, including marginal lands. An understanding of the molecular basis of their biology would contribute to better management and sustainable production. Thus, we describe the Herbivore Transcriptome Integrated Resource (HTIR), an integrated transcriptome resource (https://yanglab.hzau.edu.cn/HTIR#/). The HTIR comprises newly generated transcriptomic data from 100 to 105 tissues from two female herbivores from seven species (cattle, sheep, goats, sika deer, horses, donkeys, and rabbits) and two breeds of sheep. The HTIR houses extra 34,326 published RNA-seq datasets, making it hold 54 billion high-quality reads in total with an average mapping rate of ~93.92%. The HTIR has a user-friendly interface and provides functionalities that facilitate the exploration of gene expression between tissues and species, including electronic fluorescent pictograms, co-expression, and comparative transcriptomics. The HTIR hosts other tools, including Gene index, JBrowser, Blast, Seq fetch, GO enrichment, KEGG enrichment, and Data2heatmap.