Indisulam demonstrates significant antineoplastic properties in the context of T-cell acute lymphoblastic leukemia (T-ALL). Its effectiveness is primarily due to the degradation of RBM39, attenuating cell proliferation, promoting apoptosis and interfering with cell cycle progression in vitro, while facilitating tumor remission in T-ALL in vivo models. Overall design: mRNA profiles of J.gamma1 cells treated with 5μM indisulam or DMSO for 16h were generated by RNA sequencing, in double, using Illumina NovaSeq6000.