Next generation sequencing is a time saving and cost-efficient method to detec the complete mitochondrial genome compared to Sanger sequencing. MPS-generated full mtDNA genome sequences may have great utility in forensic real casework applications, with a pronounced gain of genetic information and discrimination power provided by coding region evaluation and the enhanced capacity of heteroplasmies determination. In this study, Shandong Han population is the first complete mtGenome dataset contributed to the database from East Asian. This will help to overcome the barriers to wider implementation of complete mtDNA typing in forensics.