Our study utilizes whole transcriptome analysis via microarray technology to uncover non-coding RNA regulators and hub genes within sperm samples afflicted by non-obstructive azoospermia. This comprehensive approach offers insight into the intricate molecular landscape underlying this condition, shedding light on potential regulatory mechanisms and key genetic players implicated in its pathogenesis. Overall design: We evaluate RNA transcripts to identify hub gene and non-coding RNA regulators in sperm of three cases of non-obstructive azoospermia and 3 controls by microarray