Black seed pumpkin is a unique vegetable resource in Yunnan Province, which has excellent stress resistance and disease resistance, especially for melon Fusarium wilt like disease has high resistance. However, there are many local varieties of black seed pumpkin, with large morphological differences and chaotic production,The quality is good or bad, and there are great differences in Fusarium wilt resistance. To evaluate the resistance difference of black seed pumpkin to Fusarium wilt,Obtaining high resistance germplasm and mining resistance genes of Fusarium wilt are the premise of effective utilization of this resource.