A novel strain isolated from Soil, and identified as Bacillus amyloliquefaciens MK2, could control the postharvest disease gray mold in strawberries. An in-vitro investigation showed that MK2 had significant bioactivity against Botrytis cinerea, with an observed zone of inhibition of 85%. The strain MK2 was 88% effective in controlling gray mold on detached fruits. De novo whole genome sequencing analysis showed that strain MK2 has a single circular chromosome with a genome size of 736,465 bp, a G+C content of 66.34%, a coding ratio of 89.80, and a protein-coding gene of 442. The NR database identified about 2296 genes among Bacillus spp. and B. amyloliquefaciens, sharing the maximum number of 649 genes with the MK2 strain.