To explore the effects of moderate intensity exercise on protein of lactylation in mouse muscle tissue metabolism. The healthy adult mice running for 6 weeks as exercise model and sedentary mice as control were used to perform transcriptomic, proteomic, lactylation-proteomics, and metabolomics analysis. In addition, correlation analysis between transcriptome and proteome, and proteome and metabolome was conducted as well. Overall design: To explore the effects of moderate intensity exercise on protein of lactylation in mouse muscle tissue metabolism. The 3 healthy adult mice running for 6 weeks as exercise model and 3 sedentary mice as control were used to perform transcriptomic, proteomic, lactylation-proteomics, and metabolomics analysis.