CTC-generated tumor tissues were interrogated at single-cell gene and protein expression levels using 10x Genomics Xenium Overall design: Tissue sections were processed using the manufacturer?s guidelines for Xenium (10x Genomics; www.10xgenomics.com). Both the standard pre-designed human multi-tissue/cancer panel (10x Genomics, product number 1000626), as well as a custom designed panel of 22 genes were used to generate the 10x Xenium results.!Series_type = Other