Spine grapes (Vitis davidii Foex) are one of the most typical wild grape resources, originating from China. Its propagation mainly depends on cutting, but rooting is difficult to overcome in production. Therefore, the purpose of this study was to analyze the mechanism of hormone affecting rooting of cutting. We select four states during the process of grape cutting: early cutting stage (P1), leaf spreading stage (P2), callus formation stage (P3), and early root generation stage (P4) to conduct transcriptome sequencing,